Interspecies differences in sensitivity to chemical exposures pose a great challenge in toxicological risk assessments. How an organism copes with chemicals is largely determined by the genes and proteins that collectively function to defend against, detoxify and eliminate chemical stressors. This integrative network includes receptors and transcription factors, biotransformation enzymes, transporters, antioxidants, and metal- and heat-responsive genes, and is collectively known as the chemical defensome. Although the types of defensome genes are generally conserved in animals, there are important differences in the complement and function of specific genes between species. Being the largest group of vertebrate species, teleost fish can provide valuable insight into the evolution and functional diversity of defensome genes.
In this study, we compared the genes comprising the chemical defensome of five fish species that span the teleosteii evolutionary branch and are often used as model species in toxicological studies and environmental monitoring programs, including Zebrafish (Danio rerio), Atlantic cod (Gadus morhua), medaka (Oryzias latipes), Atlantic killifish (Fundulus heteroclitus) and stickleback (Gasterosteus aculeatus). Genome mining revealed evolved differences in the number and composition of defensome genes that can have implication for how the species sense and respond to environmental pollutants. Furthermore, we show that there are important differences in expression of chemical defense genes between stickleback and zebrafish during early development. The results indicate that the diversity and function of the defensome will be important for toxicological testing and risk assessments studies.
SEEK ID: https://fairdomhub.org/studies/424
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Projects: Systems toxicology of Atlantic cod
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Jared V. Goldstone, John Stegeman
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Created: 22nd Oct 2018 at 09:35
Last updated: 16th Dec 2020 at 22:47
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Projects: Systems toxicology of Atlantic cod, DigiSal
Institutions: University of Bergen
Expertise: exposure studies, ex vivo, in vivo, genome mining
Projects: Systems toxicology of Atlantic cod
Institutions: University of Bergen
https://orcid.org/0000-0003-4110-0748Projects: Systems toxicology of Atlantic cod
Institutions: University of Bergen
Projects: Systems toxicology of Atlantic cod
Institutions: University of Bergen
https://orcid.org/0000-0003-4684-317XThe goal of the dCod-project is to combine the competencies in environmental toxicology, biology, bioinformatics and mathematics across the traditional department boundaries, to create a deeper understanding of cods' adaptations and reactions to stressors in the environment. Building on the thoroughly studies and mapping of the cod genome at UiO and the long research traditions on cod at the Department of Biology at UiB, the dCod project will expand our knowledge with methods based on genomics; ...
Projects: Systems toxicology of Atlantic cod
Web page: http://dcod.no
Coastal zones and oceans constitute an essential fundament for Norway’s history as well as current economy, providing ecosystem services for fisheries, aquaculture, transport, tourism, and recreation. The petroleum activities in Norwegian waters have been crucial for Norway’s financial growth and in financing the Norwegian welfare state. As the pressure on the oceans continuously increases, both the petroleum industry and the seafood industries have acknowledged that awareness and actions are ...
Programme: dCod 1.0: decoding systems toxicology of cod
Public web page: http://www.uib.no/en/dcod
Organisms: Gadus morhua
Build the chemical defensome gene list for 5 fish: Zebrafish (Danio rerio), Atlantic cod (Gadus morhua), medaka (Oryzias latipes), Atlantic killifish (Fundulus heteroclitus) and stickleback (Gasterosteus aculeatus). Source code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/defensomeGenes
Submitter: Xiaokang Zhang
Biological problem addressed: Stress response/Adaptation
Investigation: 1 hidden item
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: Chemical defensome genes overview for five fish, Chemical defensome relevant Pfam accession list, Gene pattern to filter defensome relevant genes
Snapshots: No snapshots
To study the defensome genes' expression in early developmental stages of zebrafish and stickleback. Souce code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/developmentalStages
Submitter: Xiaokang Zhang
Biological problem addressed: Stress response/Adaptation
Investigation: 1 hidden item
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: Defensome gene expression in early develepmenta..., Defensome gene expression in early develepmenta..., Gene lists for clusters in developmental stages..., Gene lists for clusters in developmental stages...
Snapshots: Snapshot 1, Snapshot 2
Exposing zebrafish to benzo(a)pyrene (B(a)P) (gene counts from NCBI GEO: GSE64198, previously published by Fang, et al. 2015. Souce code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/exposureResponse
Submitter: Xiaokang Zhang
Biological problem addressed: Stress response/Adaptation
Investigation: 1 hidden item
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: Exposure response on defensome genes in zebrafi...
Snapshots: No snapshots
Creator: Xiaokang Zhang
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Creator: Xiaokang Zhang
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Investigations: 1 hidden item
Studies: The chemical defensome of fish
More information can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/developmentalStages/zebrafish
Creator: Xiaokang Zhang
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Source code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/developmentalStages/stickleback
Creator: Xiaokang Zhang
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Creators: Xiaokang Zhang, Marta Eide, Odd André Karlsen, Inge Jonassen, Anders Goksøyr, Jared V. Goldstone; John Stegeman
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
There are four datasheets in the Excel file: 1. Gene pattern and the corresponding category (the gene list is then divided into the other three datasheets); 2. These gene names (patterns) can be directly followed by either a letter or a number; 3. These gene names (patterns) should be directly followed by a letter; 4. These gene names (patterns) should be directly followed by a number.
Creators: Xiaokang Zhang, Marta Eide, Odd André Karlsen, Inge Jonassen, Anders Goksøyr, Jared V. Goldstone; John Stegeman
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Creators: Xiaokang Zhang, Marta Eide, Odd André Karlsen, Inge Jonassen, Anders Goksøyr, Jared V. Goldstone; John Stegeman
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Abstract (Expand)
Authors: Marta Eide, Xiaokang Zhang, Odd André Karlsen, Jared V. Goldstone, John Stegeman, Inge Jonassen, Anders Goksøyr
Date Published: 1st Dec 2021
Publication Type: Journal
DOI: 10.1038/s41598-021-89948-0
Citation: Sci Rep 11(1),10546
Supplementary Tables 1-4 for manuscript "The chemical defensome of five model teleost fish"
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Creator: Xiaokang Zhang
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Creator: Xiaokang Zhang
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish
Supplementary Table1 and Table2 for manuscript "The chemical defensome of fish"
Creators: Xiaokang Zhang, Marta Eide, Inge Jonassen, Anders Goksøyr, Odd André Karlsen, Jared V. Goldstone; John Stegeman
Submitter: Xiaokang Zhang
Investigations: 1 hidden item
Studies: The chemical defensome of fish