D39
D39
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wild-type
wild-type
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Created: 8th Feb 2011 at 11:58
Last updated: 19th Oct 2012 at 15:57
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Projects: Noisy-Strep
Institutions: University of Groningen
The Veening lab is interested in phenotypic bi-stability in Streptococcus pneumoniae and its importance in virulence of this human pathogen.
Cells were grown to mid-exponential phase (OD600nm ~0.2) in GM17 medium at 37°C (with 0.15 mM ZnSO4 where relevant) and 84 ml of culture was mixed by inverting with 8.4 ml of fixing solution (50 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM EGTA, 1 mM EDTA, 30% (v/v) formaldehyde) and incubated at room temperature for 30 min. Cells were disrupted and crosslinked DNA was sheared by sonication. Antibodies coupled to magnetic beads were used to pull down cross-linked complexes. DNA was purified, amplified, ...
Submitter: Jan-Willem Veening
Assay type: Transcriptomics
Technology type: ChIP-on-chip
Investigation: Wetlab approach to transcription fidelity
