V583
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wild-type
wild-type
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Created: 28th Apr 2010 at 16:21
Last updated: 19th Oct 2012 at 15:57

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Institutions: University of Amsterdam
Expertise: Microbiology, Biochemistry, Molecular Biology, Escherichia coli, HPLC
Tools: Biochemistry, Molecular Biology, Fermentation, Chromatography
Martijn Bekker (1979) was born in Amstelveen (The Netherlands). He started his studies in biology in 1997 at the University of Amsterdam, and graduated in 2003 with specializations in molecular microbiology and in immunology. The internships during his undergraduate studies were carried out in the labs of Prof. dr. B. Oudega (VU, Amsterdam, The Netherlands) and Prof. dr. F. Heffron (OHSU, Portland, Oregon, USA). He continued with his graduate studies in 2003 in the Laboratory for Molecular Microbial ...
Measurements on Km, Vmax and allosteric activation or inhibition of the heterologously expressed (E. coli) and purifiied main L-lactate dehydrogenase
Submitter: Martijn Bekker
Assay type: Enzymatic Assay
Technology type: Enzymatic Activity Measurements
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactobacillus plantarum : WCFS1 (wild-type / wild-type), Enterococcus faecalis : V583 (wild-type / wild-type), Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: Measurement of LDH activity
Data files: The effects of fructose-1,6-bisphosphate, phosp...
Snapshots: No snapshots
L. lactis, E. faecalis and S. pyogenes are referred to as LAB because of the fact that in the presence of glucose lactate is produced as the main fermentation product . This metabolic pathway is relatively inefficient, since only 2 ATP are generated from one glucose molecule. All three LAB possess the genetic make up for mixed acid fermentation, a more effective way of fermentation generating 3 ATP per molecule of glucose. All three genomes reveal (at least) two genes encoding a lactate dehydrogenase ...
Submitter: Martijn Bekker
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactococcus lactis, Streptococcus pyogenes : M49 (591) (wild-type / wild-type), Enterococcus faecalis : V583 (wild-type / wild-type)
SOPs: No SOPs
Data files: Maximal specific growth rates of the three lact...
Snapshots: No snapshots
L. lactis, S. pyogenes and E. faecalis were grown in C-limited chemostat cultures at various pH's and dilution rates. General flux distribution, yields and other physiological factors were studied.
Submitter: Martijn Bekker
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactococcus lactis, Streptococcus pyogenes : M49 (591) (wild-type / wild-type), Enterococcus faecalis : V583 (wild-type / wild-type)
SOPs: No SOPs
Data files: Characterization of flux distribution and gener..., Characterization of flux distribution and gener..., Characterization of flux distribution and gener...
Snapshots: No snapshots
In this experiment we glucose-pulsed an E. faecalis cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catqabolism of E. faecalis
Submitter: Martijn Bekker
Assay type: Metabolomics
Technology type: Progressive Curve Experiment
Investigation: The Attic
Organisms: Enterococcus faecalis : V583 (wild-type / wild-type)
SOPs: SOP 6 Analysis of intracellular metabolites, SOP 7 Glucose-pulse experiments
Data files: No Data files
Snapshots: No snapshots
Metabolic network of Enterococcus faecalis including primary metabolism, polysaccharide metabolism, purine and pyrimidine biosoynthesis, teichoic acid biosynthesis, fatty acid and phospholipid bioynthesis, amino acid metabolism, vitamins and cofactors
Submitter: Nadine Veith
Biological problem addressed: Metabolic Network
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Enterococcus faecalis : V583 (wild-type / wild-type)
Models: Genome-scale metabolic model of Enterococcus fa...
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots