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Created: 8th Feb 2011 at 13:03
Last updated: 19th Dec 2012 at 09:59
Last used: 3rd Mar 2021 at 17:02

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Projects: Noisy-Strep
Institutions: University of Groningen
Roles: Project Coordinator
Expertise: Microbiology, Genetics, Molecular Biology, Single Cell analysis, Bacillus subtilis, Bacterial Cell Biology, Molecular microbiology, Medical microbiology, Streptococcus pneumoniae
Tools: Microbiology, Biochemistry, Genetics, Genetic modification, Transcriptomics, Fluorecence based reporter gene analyses/single cell analyses, Molecular biology techniques (RNA/DNA/Protein)
The Veening lab is interested in phenotypic bi-stability in Streptococcus pneumoniae and its importance in virulence of this human pathogen.
Bistable switches are the key elements of the regulatory networks governing cell development, differentiation and life-strategy decisions. Transcriptional noise is a main determinant that causes switching between different states in bistable systems. By using the human pathogen Streptococcus pneumoniae as a model bacterium, we will investigate how transcriptional fidelity and processivity influence (noisy) gene expression and participate in bistability. To study this question, we will use both
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Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=163
Organisms: Streptococcus pneumoniae
Handling and manipulation of S. pneumoniae using molecular, cell biological and genetic tools.
Snapshots: No snapshots
Studies: Automated time-lapse microscopy, Chromosome segregation in S. pneumoniae, Investigation of bacterial transcription fidelity and processivity, The role of transcription factor GreA in transcription fidelity and proc...
Assays: Kinetics of misincorporation and proofreading by bacterial RNA polymerase, Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae usin..., ParB-GFP ChIP on chip, RNA-Seq
Here we develop a set of new tools for S. pneumoniae and as a case study we show that S. pneumoniaea SMC is recruited to oriC by ParB and promotes chromosome segregation.
Person responsible: Jan-Willem Veening
Snapshots: No snapshots
Investigation: Wetlab approach to transcription fidelity
Assays: ParB-GFP ChIP on chip
Cells were grown to mid-exponential phase (OD600nm ~0.2) in GM17 medium at 37°C (with 0.15 mM ZnSO4 where relevant) and 84 ml of culture was mixed by inverting with 8.4 ml of fixing solution (50 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM EGTA, 1 mM EDTA, 30% (v/v) formaldehyde) and incubated at room temperature for 30 min. Cells were disrupted and crosslinked DNA was sheared by sonication. Antibodies coupled to magnetic beads were used to pull down cross-linked complexes. DNA was purified, amplified,
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Submitter: Jan-Willem Veening
Assay type: Transcriptomics
Technology type: ChIP-on-chip
Snapshots: No snapshots
Investigation: Wetlab approach to transcription fidelity
Study: Chromosome segregation in S. pneumoniae
Organisms: Streptococcus pneumoniae : D39 (wild-type / wild-type) (batch)
SOPs: No SOPs
Data files: ChIP-chip ParB-GFP