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Created: 20th Feb 2011 at 20:11
Last updated: 22nd Nov 2011 at 20:12
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Projects: Noisy-Strep
Institutions: University of Newcastle
Bistable switches are the key elements of the regulatory networks governing cell development, differentiation and life-strategy decisions. Transcriptional noise is a main determinant that causes switching between different states in bistable systems. By using the human pathogen Streptococcus pneumoniae as a model bacterium, we will investigate how transcriptional fidelity and processivity influence (noisy) gene expression and participate in bistability. To study this question, we will use both
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Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=163
Organisms: Streptococcus pneumoniae
Handling and manipulation of S. pneumoniae using molecular, cell biological and genetic tools.
Snapshots: No snapshots
Studies: Automated time-lapse microscopy, Chromosome segregation in S. pneumoniae, Investigation of bacterial transcription fidelity and processivity, The role of transcription factor GreA in transcription fidelity and proc...
Assays: Kinetics of misincorporation and proofreading by bacterial RNA polymerase, Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae usin..., ParB-GFP ChIP on chip, RNA-Seq
Artificial transcription elongation compexes are assembled in vitro using synthetic deoxy-oligonucleotides (representing template and non template DNA strands), radiolabelled RNA (representing nascent transcript) and purified RNA polymerase. After high salt wash the incorrect NTP is added and reaction is allowed to proceed for the various amounts of time. Reaction is stopped by addition of formamide-containing loading solution and the products are resolved on high-percentage denaturing polyacryamide
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Submitter: Nikolay Zenkin
Assay type: Reactomics
Technology type: Enzymatic Activity Measurements
Snapshots: No snapshots
Investigation: Wetlab approach to transcription fidelity
Study: Investigation of bacterial transcription fideli...
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files