Nikolay Zenkin
SEEK ID: https://fairdomhub.org/people/344
Location:
United Kingdom
ORCID: Not specified
Joined: 17th Feb 2011
Their tagsRelated items
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Bistable switches are the key elements of the regulatory networks governing cell development, differentiation and life-strategy decisions. Transcriptional noise is a main determinant that causes switching between different states in bistable systems. By using the human pathogen Streptococcus pneumoniae as a model bacterium, we will investigate how transcriptional fidelity and processivity influence (noisy) gene expression and participate in bistability. To study this question, we will use both ...
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=163
Submitter: Nikolay Zenkin
Investigation: Wetlab approach to transcription fidelity
Assays: Kinetics of misincorporation and proofreading by bacterial RNA polymerase
Artificial transcription elongation compexes are assembled in vitro using synthetic deoxy-oligonucleotides (representing template and non template DNA strands), radiolabelled RNA (representing nascent transcript) and purified RNA polymerase. After high salt wash the incorrect NTP is added and reaction is allowed to proceed for the various amounts of time. Reaction is stopped by addition of formamide-containing loading solution and the products are resolved on high-percentage denaturing polyacryamide ...
Submitter: Nikolay Zenkin
Assay type: Reactomics
Technology type: Enzymatic Activity Measurements
Investigation: Wetlab approach to transcription fidelity
Abstract (Expand)
Authors: , , M. Herber, L. Attaiech, , , S. Klumpp, ,
Date Published: 6th Sep 2014
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Authors: , Mohammad Roghanian,
Date Published: 10th Jul 2012
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Date Published: 3rd Jan 2012
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Authors: Aleksandra Bochkareva, , Vasisht R Tadigotla,
Date Published: 29th Nov 2011
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Authors: , Vasisht R Tadigotla, Konstantin Severinov,
Date Published: 26th Jul 2011
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Authors: Mohammad Roghanian, ,
Date Published: 27th Jan 2011
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Date Published: 1st Jun 2010
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Authors: , Aleksandra Bochkareva, Vasisht R Tadigotla, Mohammad Roghanian, Savva Zorov, Konstantin Severinov,
Date Published: 1st Apr 2010
Publication Type: Not specified
