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Sulman Shafeeq

About Sulman Shafeeq:
No description specified

SEEK ID: https://fairdomhub.org/people/409

Location: Netherlands

Expertise: Transcriptomics, Microarray analysis, carbon metabolism, gene regulation, Streptococcus pneumoniae Molecular microbiology, Bacterial Cell Biology, Genetics

Tools: Transcriptomics, Microbiology, Molecular biology techniques (RNA/DNA/Protein), Microarray analysis

ORCID: Not specified

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Project positions

Noisy-Strep (University of Groningen)

Related items

Noisy-Strep

Bistable switches are the key elements of the regulatory networks governing cell development, differentiation and life-strategy decisions. Transcriptional noise is a main determinant that causes switching between different states in bistable systems. By using the human pathogen Streptococcus pneumoniae as a model bacterium, we will investigate how transcriptional fidelity and processivity influence (noisy) gene expression and participate in bistability. To study this question, we will use both
...

Programme: SysMO

Public web page: http://www.sysmo.net/index.php?index=163

Control of transcription elongation by GreA determines rate of gene expression in Streptococcus pneumoniae
Noisy-Strep

Abstract (Expand)

Transcription by RNA polymerase may be interrupted by pauses caused by backtracking or misincorporation that can be resolved by the conserved bacterial Gre-factors. However, the consequences of such … pausing in the living cell remain obscure. Here, we developed molecular biology and transcriptome sequencing tools in the human pathogen Streptococcus pneumoniae and provide evidence that transcription elongation is rate-limiting on highly expressed genes. Our results suggest that transcription elongation may be a highly regulated step of gene expression in S. pneumoniae. Regulation is accomplished via long-living elongation pauses and their resolution by elongation factor GreA. Interestingly, mathematical modeling indicates that long-living pauses cause queuing of RNA polymerases, which results in 'transcription traffic jams' on the gene and thus blocks its expression. Together, our results suggest that long-living pauses and RNA polymerase queues caused by them are a major problem on highly expressed genes and are detrimental for cell viability. The major and possibly sole function of GreA in S. pneumoniae is to prevent formation of backtracked elongation complexes.

Authors: Yulia Yuzenkova, Pamela Gamba, M. Herber, L. Attaiech, Sulman Shafeeq, Oscar Kuipers, S. Klumpp, Nikolay Zenkin, Jan-Willem Veening

Date Published: 6th Sep 2014

Journal: Nucleic Acids Res

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