Ion Flux Changes of Potassnium Uptake Investigation
How does the flux of potassium, hydrogen change during potassium uptake? Which ions are involved in maintaining the required charge balance?
SEEK ID: https://fairdomhub.org/studies/44
Potassium uptake in Saccharomyces cerevisiae
Projects: TRANSLUCENT
Experimentalists: Not specified
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Created: 3rd Jun 2010 at 11:55
Last updated: 22nd Nov 2011 at 20:12
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Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
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Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
Programme: SysMO
Public web page: http://www.translucent-network.org/
Experimental approaches to study the mechanism and ions involved in potassium uptake after long term potassium starvation.
Snapshots: No snapshots
The potassium influx after the addition of a certain amount of KCl to a potassium free medium, followed by the injection of glucose will be measured by using the MIFE and FLISE technique. This reveals a time course of potassium. Also the external potassium concentrations will be measured.
Contributor: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
In parallel with the potassium influx the efflux of protons is monitored by measuring the external proton concentration changes with MIFE or FLISE.
Contributor: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
Further fluxes (ammonium, chloride, calcium) will be measured dependent on the capacities of the MIFE/FLISE technique.
Contributor: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
