Assays

Semi-quantitative determination of Cyp1a protein amount in cod liver, using the ELISA method.

Kinetic characterisation en mathematical modelling of ENO.

Batch cultures of designed media inocculated with biomass from WWTP anaerobious fermentation steps. The media consisted of a series of macro- and micro-nutrients to avoid limitations on proliferation, and a fermentative substrate of glucose and aminoacids. The glucose proportion and aminoacid composition varied culture to culture according to previous work using a mathematical model of a mixed culture fermentation in similar conditions. Acetoclastic methanogenesis was avoided with BES at 1 ...

Enzyme activity of glutathione-s-transferase (Gst) and catalase (Cat) in Kollevåg samples

Enzyme activity of the oxidative stress enzymes glutathione S-transferase (Gst) and catalase (Cat) measured in cod liver

Based on initial cell material, series of SOPs connected with assays of enzymes involved in Central Carbon Metabolism of S. solfataricus have been developed.

Concentrations of steroid hormones E2 and T in female cod plasma from Kollevåg fish

Cyp1a activity (=EROD) measured in liver samples of cod exposed to chlorpyrifos-methyl

Data and Python scripts to run the analysis of literature data that estimates rates of protein synthesis in the light and dark, and overall rates of protein turnover, in Cyanothece and Ostrecoccus tauri.

HSD11B1 was inhibited by CBX and conversion of cortisone and 11KA4 by HSD11B1 and AKR1C3 was followed over time.

Collection for experimental SOPs

Experimental data for the conversion of 3PG to F6P and the gluconeogenic pathway intermediates

Exposing zebrafish to benzo(a)pyrene (B(a)P) (gene counts from NCBI GEO: GSE64198, previously published by Fang, et al. 2015. Souce code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/exposureResponse

To study the defensome genes' expression in early developmental stages of zebrafish and stickleback. Souce code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/developmentalStages

Model outputs mRNA expression of PIF4/5 that is under control of the Pokhilko extended circadian clock. The first version (Model 2a in the supplementary file) has inhibition of PIFs from TOC1. The second version (Model 2c) has PIF activity promoted by LHY/CCA1 - this is currently the most accurate model when compared to data. Models shall be updated later to include PIF4/5 protein levels. Parameter values for this and other External Coincidence models found in supplementary file.Originally submitted ...

Model outputs mRNA expression of PIF4/5 that is under control of the Pokhilko extended circadian clock. The first version (Model 2a in the supplementary file) has inhibition of PIFs from TOC1. The second version (Model 2c) has PIF activity promoted by LHY/CCA1 - this is currently the most accurate model when compared to data. Models shall be updated later to include PIF4/5 protein levels. Parameter values for this and other External Coincidence models found in supplementary file.Version Comments ...

External pH changes for the conditions of Navarrete et al. (2010) will be estimated from the internal pH changes and the volume ratio of cell sample to medium.

External concentration changes under the conditions of Navarrete et al. (2010) will be estimated from the internal concentration changes and the volume ratio of cell sample to medium.

extracellular metabolite concentrations measured by 1H-NMR