Analysis of Central Carbon Metabolism of Sulfolobus solfataricus under varying temperature

The Sulfolobus systems biology (‘‘SulfoSYS’’)-project represented the first (hyper-)thermophilic Systems Biology project, funded within the European trans-national research initiative ‘‘Systems Biology of Microorganisms’’. Within the SulfoSYS-project, focus lies on studying the effect of temperature variation on the central carbohydrate metabolism (CCM) of S. solfataricus that is characterized by the branched Entner–Doudoroff (ED)-like pathway for sugar (glucose, galactose) degradation and the Embden–Meyerhof–Parnas (EMP)-like pathway, which is employed during gluconeogenesis. The effect of temperature changes on the CCM network of S. solfataricus is analyzed by the tight integration of bioinformatics, genome, transcriptome, proteome, metabolome, and enzymatic data, with all –omic and biochemical data being produced from identical batches of biomass. Beside providing experimental data, one main part of this highly integrative project is the in silico analysis of the CCM network, including the design of a sufficiently precise model according to the silicon cell type model . This model will allow for the computation of the S. solfataricus CCM, and in particular to investigate its robustness to changes in temperature at the system level.

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Created: 23rd Sep 2009 at 16:14

Last updated: 22nd Nov 2011 at 20:12

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