Batch cultures of designed media inocculated with biomass from WWTP anaerobious fermentation steps. The media consisted of a series of macro- and micro-nutrients to avoid limitations on proliferation, and a fermentative substrate of glucose and aminoacids. The glucose proportion and aminoacid composition varied culture to culture according to previous work using a mathematical model of a mixed culture fermentation in similar conditions. Acetoclastic methanogenesis was avoided with BES at 1 g/L and all media were buffered.
Product concentrations were determined through Gass Chromatography --for Volatile Fatty Acids-- and High Performance Liquid Chromatography --for other products and remaining glucose--.
SEEK ID: https://fairdomhub.org/assays/2307
Experimental assay
Projects: ODDITY
Investigation: hidden item
Study: hidden item
Assay position:
Assay type: Metabolite Concentration
Technology type: Chromatography
Organisms: No organisms
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Created: 11th Mar 2024 at 10:14
Last updated: 11th Mar 2024 at 18:18