Enrichment of anaerobious mixed cultures in favor of odd-chain volatile fatty acid production
Batch cultures of designed media inocculated with biomass from WWTP anaerobious fermentation steps. The media consisted of a series of macro- and micro-nutrients to avoid limitations on proliferation, and a fermentative substrate of glucose and aminoacids. The glucose proportion and aminoacid composition varied culture to culture according to previous work using a mathematical model of a mixed culture fermentation in similar conditions. Acetoclastic methanogenesis was avoided with BES at 1 g/L and all media were buffered.
Product concentrations were determined through Gass Chromatography --for Volatile Fatty Acids-- and High Performance Liquid Chromatography --for other products and remaining glucose--.
SEEK ID: https://fairdomhub.org/assays/2307
Experimental assay
Projects: ODDITY
Investigation: hidden item
Study: hidden item
Assay position:
Assay type: Metabolite Concentration
Technology type: Chromatography
Organisms: No organisms
Export PNG
Download
Creators and Submitter
Views: 118
Created: 11th Mar 2024 at 10:14
Last updated: 11th Mar 2024 at 18:18
Related items
Projects: ODDITY
Institutions: Universidade de Santiago de Compostela
https://orcid.org/0009-0004-0159-7539
Biorefineries are set to become an important agent in the shift towards a circular economy due to their potential to valorize organic wastes into marketable products. Anaerobic fermentations yielding volatile fatty acids (VFA) are a key process in this biorefinery paradigm as VFA act as intermediates between the organic wastes and the final biorefinery products. However, the development of a new bioprocess based on mixed-culture fermentation is an extremely challenging task. Targeting the desired ...
Projects: ODDITY, WATCHER, VIALACTEA
Web page: https://biogroup.usc.es/programme32
Model-based design of a biorefinery platform to valorise agrifood organic waste into odd-chain volatile fatty acids integrating technical and environmental criteria
The conversion of organic wastes by anaerobic fermentation (the so-called carboxylate platform) is one of the promising emerging biorefineries that allows valorising organic carbon to volatile fatty acids (VFA), which can be further processed as chemicals, biopolymers and biofuels. A major obstacle to the widespread of the carboxylate ...
Programme: Targeted VFA production from (waste) waters - Biogroup
Public web page: https://biogroup.usc.es/ODDITY
Start date: 1st Dec 2022
End date: 30th Nov 2024
Culture conditions along with substrate and product compositions throughout the culture.
