PFK-1 and PFK-2 were cloned and expressed in E. coli, purified, and kinetically characterised in terms of substrates and allosteric regulators.
SEEK ID: https://fairdomhub.org/studies/1252
ADP dependent cyanobacterial PFK-A
Projects: SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent De...
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Created: 19th Feb 2024 at 11:09
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Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory Layers, Project Coordination, WP 3: Drug release kinetics study, Glucose metabolism in cancer cell lines
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University
The Deutsche Forschungsgemeinschaft (DFG) is the self-governing organisation for science and research in Germany. It serves all branches of science and the humanities. In organisational terms, the DFG is an association under private law. Its membership consists of German research universities, non-university research institutions, scientific associations and the Academies of Science and the Humanities.
Web page: https://www.dfg.de/en/index.jsp
Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Theyhad and have tremendous influence on the biogeochemical cycles on Earth. Recently,cyanobacteria are increasingly investigated as cell factories for a sustainableeconomy. Despite their global, environmental and rising economic importance, ourknowledge on the regulation of their primary metabolism is fragmented.Cyanobacteria switch between photoautotrophic and heterotrophic modes ofmetabolism during day/night cycles or ...
Programme: DFG founded projects
Public web page: https://gepris.dfg.de/gepris/projekt/397695561
Start date: 1st Sep 2018
Organisms: Synechocystis sp. PCC 6803
Cyanobacterial PFKs were thought to be ATP dependent, but isolation and characterisation of 2 PFK isoenzymes from Synechocystis revealed that they belong to the PFK-A family, use ADP as phosphate donor and form a separate phylogenetic class. Their allosteric regulation via 3-PG and ATP respectively allow for flexible switching between aactive and inactive enzymes dependent on the light and carbon status.
The purified enzymes were kinetically charactyerised in terms of substrate sensitivity and alloestric regulation.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: ADP dependent cyanobacterial PFK-A
Study: PFK-1 and PFK-2 kinetics
Organisms: No organisms
SOPs: No SOPs
Data files: Kinetic data for PFK-1 and PFK-2
Snapshots: No snapshots
The experimental data for PFK-1 and PFK-2 were analysed and fitted with a MWC equation. The model is described in Mathematica and the plots in the manuscript are generatedin the notebook.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: ADP dependent cyanobacterial PFK-A
Study: PFK-1 and PFK-2 kinetics
Organisms: No organisms
Models: Mathematica notebook for enzyme kinetic data an...
SOPs: No SOPs
Data files: Kinetic data for PFK-1 and PFK-2
Snapshots: No snapshots
Experimental data for kinetic characterisaton of PFK-1 and PFK-2.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Mathematic notebook for enzyme kinetic analysis and model fit, including figure generation for manuscript.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Not specified
Model format: Mathematica
Environment: Mathematica
Organism: Synechocystis sp. PCC 6803
Investigations: ADP dependent cyanobacterial PFK-A
Studies: PFK-1 and PFK-2 kinetics