Experimental data for enzyme kinetic characterisation of PFK-1 and PFK-2
The purified enzymes were kinetically charactyerised in terms of substrate sensitivity and alloestric regulation.
SEEK ID: https://fairdomhub.org/assays/2294
Experimental assay
Projects: SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent De...
Investigation: ADP dependent cyanobacterial PFK-A
Study: PFK-1 and PFK-2 kinetics
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Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 19th Feb 2024 at 11:12
Last updated: 26th Oct 2024 at 11:51
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Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory Layers, Project Coordination, WP 3: Drug release kinetics study, Glucose metabolism in cancer cell lines
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University
The Deutsche Forschungsgemeinschaft (DFG) is the self-governing organisation for science and research in Germany. It serves all branches of science and the humanities. In organisational terms, the DFG is an association under private law. Its membership consists of German research universities, non-university research institutions, scientific associations and the Academies of Science and the Humanities.
Web page: https://www.dfg.de/en/index.jsp
Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Theyhad and have tremendous influence on the biogeochemical cycles on Earth. Recently,cyanobacteria are increasingly investigated as cell factories for a sustainableeconomy. Despite their global, environmental and rising economic importance, ourknowledge on the regulation of their primary metabolism is fragmented.Cyanobacteria switch between photoautotrophic and heterotrophic modes ofmetabolism during day/night cycles or ...
Programme: DFG founded projects
Public web page: https://gepris.dfg.de/gepris/projekt/397695561
Start date: 1st Sep 2018
Cyanobacterial PFKs were thought to be ATP dependent, but isolation and characterisation of 2 PFK isoenzymes from Synechocystis revealed that they belong to the PFK-A family, use ADP as phosphate donor and form a separate phylogenetic class. Their allosteric regulation via 3-PG and ATP respectively allow for flexible switching between aactive and inactive enzymes dependent on the light and carbon status.
PFK-1 and PFK-2 were cloned and expressed in E. coli, purified, and kinetically characterised in terms of substrates and allosteric regulators.
Experimental data for kinetic characterisaton of PFK-1 and PFK-2.
