SOP for measurement of PFK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Investigations: Glucose metabolism in Plasmodium falciparum tro...
Studies: Model construction
SOP for measurement of PGI activity in extracts.
SOP for measurement of PGK activity in extracts.
SOP for measurement of PGM activity in extracts.
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
SOP for measurement of PK activity in extracts.
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins
Creator: Miguel Curto Rubio
Contributor: The JERM Harvester
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins
Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)
Creator: Maksim Zakhartsev
Contributor: Maksim Zakhartsev
Investigations: Kinetic analysis of metabolic system using tran...
Studies: Metabolic perturbation of the steady state cult...
Assays: Cellular size and granularity during glucose pulse, Dynamics of extracellular metabolites during gl..., Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse
Method for transformation of plasmids into Lactococcus lactis
Creator: Martijn Bekker
Detection of small RNAs by blot hybridization.
Creator: Vânia Pobre
Contributor: Vânia Pobre
Creator: Jose Ramos
Describes the cultivation-, washing- and electroporation-steps required in order to transform bacterial cells with DNA.
Creator: Maximilian Bahls
Contributor: Maximilian Bahls
Investigations: 1 hidden item
Studies: 1 hidden item
Assays: 1 hidden item
The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.
Creators: Jacky Snoep, Theresa Kouril
Contributor: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Assays: FBPAase, GAPDH, PGK, TPI
Describes the steps required in order to run an error-prone PCR (epPCR) for the creation of DNA libraries of a given template gene.
Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A
Contributor: Jay Moore
Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item
Studies: Timeseries 1 and 8 hidden items
Assays: Online/offline measurements and 8 hidden items
Creators: Louise Thomas, Maggie Smith
Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Contributor: Martijn Bekker
Investigations: Investigation of glycolysis and pyruvate branch...
Studies: Comparative modeling and phosphate dependence f...
Assays: Glucose pulsed S. pyogenes
Method of how to perform the purification of glyoxalase II as well as kinetics assays.
Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.
A method of how to measure methylglyoxal present in the growth medium.
Creator: Lina Patricia Barreto Parra
Contributor: Lina Patricia Barreto Parra