SOPs

164 SOPs visible to you, out of a total of 311

SOP for measurement of PFK activity in extracts.

SOP for measurement of PGI activity in extracts.

SOP for measurement of PGK activity in extracts.

SOP for measurement of PGM activity in extracts.

SOP for measurement of PGM activity in extracts.

SOP for measurement of PK activity in extracts.

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins

Creator: Miguel Curto Rubio

Contributor: The JERM Harvester

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins

Creator: Miguel Curto Rubio

Contributor: The JERM Harvester

Method for transformation of plasmids into Lactococcus lactis

Creator: Martijn Bekker

Contributor: The JERM Harvester

Detection of small RNAs by blot hybridization.

Creator: Vânia Pobre

Contributor: Vânia Pobre

Purpose:

Creator: Jose Ramos

Contributor: The JERM Harvester

Describes the cultivation-, washing- and electroporation-steps required in order to transform bacterial cells with DNA.

Creator: Maximilian Bahls

Contributor: Maximilian Bahls

The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.

Describes the steps required in order to run an error-prone PCR (epPCR) for the creation of DNA libraries of a given template gene.

Creator: Maximilian Bahls

Contributor: Maximilian Bahls

No description specified
No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

Method of how to perform the purification of glyoxalase II as well as kinetics assays.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A method of how to measure methylglyoxal present in the growth medium.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified
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