SOPs
164 SOPs visible to you, out of a total of 311
SOP for measurement of PFK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
DownloadSOP for measurement of PGI activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins
Creator: Miguel Curto Rubio
Contributor: The JERM Harvester
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins
Creator: Miguel Curto Rubio
Contributor: The JERM Harvester
Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)
Creator: Maksim Zakhartsev
Contributor: Maksim Zakhartsev
Method for transformation of plasmids into Lactococcus lactis
Creator: Martijn Bekker
Contributor: The JERM Harvester
Describes the cultivation-, washing- and electroporation-steps required in order to transform bacterial cells with DNA.
Creator: Maximilian Bahls
Contributor: Maximilian Bahls
The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.
Creators: Jacky Snoep, Theresa Kouril
Contributor: Jacky Snoep
Describes the steps required in order to run an error-prone PCR (epPCR) for the creation of DNA libraries of a given template gene.
Creator: Maximilian Bahls
Contributor: Maximilian Bahls
Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A
Contributor: Jay Moore
Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts
Creators: Jacky Snoep, Theresa Kouril
Contributor: Jacky Snoep
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Creator: Martijn Bekker
Contributor: Martijn Bekker
Method of how to perform the purification of glyoxalase II as well as kinetics assays.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
A method of how to measure methylglyoxal present in the growth medium.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Creator: Lina Patricia Barreto Parra
Contributor: Lina Patricia Barreto Parra
