SOPs
153 SOPs visible to you, out of a total of 276
Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)
Creator: Maksim Zakhartsev
Contributor: Maksim Zakhartsev
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SOP for measurement of PFK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGI activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Creator: Lina Patricia Barreto Parra
Contributor: Lina Patricia Barreto Parra
SOP for measurement of PK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Method for transformation of plasmids into Lactococcus lactis
Creator: Martijn Bekker
Contributor: JERM
Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.
Creators: Jacky Snoep, Theresa Kouril
Contributor: Jacky Snoep
The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A
Contributor: Jay Moore
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins
Creator: Miguel Curto Rubio
Contributor: JERM
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins
Creator: Miguel Curto Rubio
Contributor: JERM
Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts
Creators: Jacky Snoep, Theresa Kouril
Contributor: Jacky Snoep
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Creator: Martijn Bekker
Contributor: Martijn Bekker
Method of how to perform the purification of glyoxalase II as well as kinetics assays.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
A method of how to measure methylglyoxal present in the growth medium.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
