This method describes the preparation of the in vivo-like buffer for the measurement of bloodstream T. brucei recombinant enzymes under pseudo-physiological conditions.
Creators: Alejandro Leroux, Luise Krauth-Siegel
Contributor: Alejandro Leroux
Investigations: Kinetic understanding of the T. brucei trypanot...
Studies: Kinetic characterization of trypanothione-depen...
Assays: Trypanothione synthetase ATP consumption steady..., Trypanothione synthetase Gsp and T(SH)2 product...
Creator: Federico Rojas
Contributor: Federico Rojas
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
This protocol is designed to prepare sufficient amounts of high-quality total
RNA from the yeast saccharomyces cerevisiae grown in liquid culture for
analysis on microarrays.
Creators: Walter Glaser, Christa Gregori
Contributor: Walter Glaser