SOPs

A kinetic model that describess the activation of a dimeric efflux system that could bind either GSH or SLG

This SOP describes the in silico ADME-Tox property prediction with QikProp for prepared pteridine ligands.

A model to describe aggregation of homomeric protein complexes in mechanosensitive channels in E.coli.

SOP for measurement of ALD activity in extracts.

SOP for growing yeast in anaerobic conditions

Describes the steps required in order to detect and quantify alcohols and other volatile compounds in microbial culture supernatants by gas chromatography with a flame-ionization detector (GC-FID).

This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.

Method for analysis of various organic acids in the medium

Measurements of K+ retained in the cytoplasm using flame photometry.

A procedure to analyse the genomic interaction of E.coli RNA polymerase (RNAP) upon methylglyoxal (MG) stress, a toxic keto-aldehyde by-product of metabolism.

Master Bash script to launch Molecular Dynamics when running the Analysis part of Simulation Foundry. Make sure you followed "Preparations" instructions! Read the Manual!

Assay methodologies for individual glycolytic isoenzymes from the Mendes Group, University of Manchester, UK

The kit is used for the quantitative detection of ATP in yeast cells by luciferase driven bioluminescence. ATP extraction is done by boiling cells in TE buffer.