SOPs
What is a SOP?Filters
Creator: Martijn Bekker
Submitter: The JERM Harvester
This method describes how to derivatize the N-glutathionylspermidine and trypanothione produced by T. brucei trypanothione synthetase under in vivo-like conditions
Creator: Alejandro Leroux
Submitter: Alejandro Leroux
This is the Jupyter Notebook to allow editing and working with the code.
Design an initial rate experiment in four steps
This script aims to help you design high-quality initial rate experiments. It uses the Michaelis-Menten equation to simulate data for an enzyme with unknown enzyme reaction parameters. Four steps are needed:
- Estimate the enzyme reaction parameters
Input: estimates of Km and vmax
and indicate the enzyme concentration concentration you are planning to use. If you have no ...
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
This is the pdf of the Jupyter Notebook to allow looking at the notebook without installing anything.
Design an initial rate experiment in four steps
This script aims to help you design high-quality initial rate experiments. It uses the Michaelis-Menten equation to simulate data for an enzyme with unknown enzyme reaction parameters. Four steps are needed: 1. Estimate the enzyme reaction parameters
Input: estimates of Km and vmax
and indicate the enzyme concentration concentration you are planning ...
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein
Creator: Femke Mensonides
Submitter: Maksim Zakhartsev
Sample preparation - SOP for sampling, preparation of cell extracts, and general assay set-up
Creator: Femke Mensonides
Submitter: Maksim Zakhartsev
The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.
Creator: Matthew Rolfe
Submitter: Matthew Rolfe
General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes
Creator: Hanan Messiha
Submitter: Maksim Zakhartsev
Describes how the DNA was isolated from salmon intestinal samples before preparations of 16S rRNA gene amplicons for the Illumina MiSeq system.
Creators: Stuart Owen, Sahar Hassani, RAGNHILD ÅNESTAD, Jesse van Dam, Dagmar Waltemath, Kristina Vagonyte-Hallan, Kristil Sundsaasen, Natalie Stanford, Lars Snipen, Sandve Simen, Jacob Seilø Torgersen, Inga Leena Angell, Dominic Nanton, Vitor Martins dos Santos, Torgeir R. Hvidsten, Thomas Harvey, Hanne Hellerud Hansen, Fabian Grammes, Arne Gjuvsland, Gareth Gillard, Graceline Tina Kirubakaran, Jon Olav Vik
Submitter: Inga Leena Angell
SOP for extracting DNA, RNA and Proteins from the mineral pellet of a bioleaching culture using hot acidic phenol. Current draft.
Creators: Malte Herold, Mario Vera, Soeren Bellenberg
Submitter: Malte Herold