Trypanothione synthetase Gsp and T(SH)2 production measured by HPLC

TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of glutathione, and spermidine.

FAIRDOMHub ID: https://fairdomhub.org/assays/246

Experimental Assay

Alejandro Leroux

Projects: SilicoTryp

Investigation: Kinetic understanding of the T. brucei trypanothione synthesis pathway

Study: Kinetic characterization of trypanothione-dependent enzymes

Assay type: Discontinuous Enzymatic

Technology type: HPLC

Organisms: No organisms

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Views: 954

Created: 6th Aug 2014 at 15:27

Last updated: 8th Nov 2017 at 15:21

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