Parasites will be harvested at different growth phases and the total amount of the proteins will be followed by western blot. The absolut concentration will be obtained by comparison with a know amount of the recombinant untagged protein. The thiol redox state of the proteins will be followed by modification of the free cys with methoxy-ethyl-maleinimide poly(ethylenglycol) (Meo-PEG-mal).
FAIRDOMHub ID: https://fairdomhub.org/studies/95
Experimentalists: Not specified
Created: 18th Jun 2012 at 17:44