Determination of the redox state and the total concentration of the trypanothione-dependent detoxification enzymes in vivo

Parasites will be harvested at different growth phases and the total amount of the proteins will be followed by western blot. The absolut concentration will be obtained by comparison with a know amount of the recombinant untagged protein. The thiol redox state of the proteins will be followed by modification of the free cys with methoxy-ethyl-maleinimide poly(ethylenglycol) (Meo-PEG-mal).

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Created: 18th Jun 2012 at 17:44

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