SOPs158 SOPs visible to you, out of a total of 293
We routinely select specific RNAi gene targets (400–600 bp) and primers using the RNAit software http://trypanofan.path.cam.ac.uk/software/RNAit.html. A single pair of PCR primers are designed that incorporate four selected restriction sites (not present in the RNAi target fragment) such that a single PCR product can be differentially digested and sequentially cloned. For example, using MCS1/2, the following primers could be used to clone antisense followed by sense fragments: Primer 1, XbaI–BamHI-5′
SOP used for detecting non influential parameters and interactions in non linear dynamical models. These parameters can be estabilished which allows the prioritization of parameters that can be subsequently estimated using robust global optimizations methods.
Written Standard Operating Procedures provide workers with the operational
information necessary to perform a job properly and ensure consistency in the
operations. Standard Operating Procedures provide a historical record of steps in
the how, why and when and serve as a training tool for teaching users.