SOPs158 SOPs visible to you, out of a total of 293
A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.
Metabolomics perturbation sample preparation and description of how the exact details of the perturbations.
-Amino acid starvation
-Oxidative stress via H2O2
-Glycerol addition to the medium