Measurement of intracellular metabolite pools upon methylglyoxal stress in E. coli using LC-MS-MS.
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A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.

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Filename: SOP_Measurements of internal SLG and GSH_Ertan_Ozyamak.pdf  Download

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Created: 26th Oct 2009 at 15:48

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