Measurement of intracellular metabolite pools upon methylglyoxal stress in E. coli using LC-MS-MS. Version 1
A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.
SEEK ID: https://fairdomhub.org/sops/22?version=1
Filename:
SOP_Measurements of internal SLG and GSH_Ertan_Ozyamak.pdf
Format: PDF document
Size: 33.8 KB
Export PNG
Creators and Submitter
Views: 2797 Downloads: 78
Created: 26th Oct 2009 at 14:48
TagsThis item has not yet been tagged.
AttributionsNone
Version History
Version 1 (earliest) Created 26th Oct 2009 at 14:48 by Lisbeth Lyngberg
No revision comments
Related items
Projects: KOSMOBAC
Institutions: University of Aberdeen
I am a research technician at the Institute of Medical Science in Aberdeen, working for Prof. Ian Booth. The topic of our workpackage deals with K+ homostasis in Escherichia coli. I am working with the protein KefF, a regulatory subunit of the potassium channel KefC.
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Ion and solute homeostasis in enteric bacteria: an integrated view generated from the interface of modelling and biological experimentation
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=56
