Measurement of intracellular metabolite pools upon methylglyoxal stress in E. coli using LC-MS-MS. Version 1
A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.
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SOP_Measurements of internal SLG and GSH_Ertan_Ozyamak.pdf
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Created: 26th Oct 2009 at 14:48
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Version 1 (earliest) Created 26th Oct 2009 at 14:48 by Lisbeth Lyngberg
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Projects: KOSMOBAC
Institutions: University of Aberdeen
I am a research technician at the Institute of Medical Science in Aberdeen, working for Prof. Ian Booth. The topic of our workpackage deals with K+ homostasis in Escherichia coli. I am working with the protein KefF, a regulatory subunit of the potassium channel KefC.
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
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