A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.
Views: 1709 Downloads: 57
Created: 26th Oct 2009 at 15:48
Last used: 22nd Oct 2020 at 08:07