Analysis of Escherichia coli strains with linear respiratory chain

A set of isogenic mutant strains was constructed which lack NADH Dehydrogenase I as well as two terminal oxidases, resulting in strains with linear respiratory chain. The different strains hence differ in the terminal oxidase and express either cytochrome bo, cytochrome bdI or cytochrome bdII.
The different strains were cultivated in glucose-limited chemostats with defined low levels of oxygen supply. Biomass and by-product formation, gene expression and the phosphorylation state of the important regulatory protein ArcA were determined and compared with the wildtype strain MG1655.

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Created: 15th May 2014 at 11:49

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