Assay type 'Metabolomics'

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42 Assays visible to you, out of a total of 53

Measurement of intra- and extra-cellular metabolome.

Contributor: Ulf Liebal

Assay type: Metabolite Profiling

Technology type: Liquid Chromatography-tandom Mass Spectrometry

Snapshots: No snapshots

Pilot experiment concerning metabolome of S. solfataricus was conducted in order to acquire SOPs regarding the technique and gain insight on differences in metabolite concentrations at 70 and 80C

Contributor: Pawel Sierocinski

Assay type: Metabolomics

Technology type: Gas Chromatography Mass Spectrometry

Snapshots: No snapshots

This document describes by-product formation rates measured in MG1655 at steady-state conditions in Infors-Multifors-Bioreactors.

S. pyogenes was grown in rich medium, strongly concentrated and glucose-pulsed in a MES buffer. Intracellular metabolite concentration is followed in time.

The task of this assay is to determine the impact of oxygen availability on the concentrations of metabolites from different central metabolic pathways. The focus lies on metabolites connected to glycolysis, tri-carbon-acid-cycle and energy metabolism. All strains have been cultured and analysed according to the SOPs listed below

experimentally measured extracellular fluxes in yeast Saccharomyces cerevisiae in anaerobic glucose limited chemostat (D=0.1 h-1) on minimal medium

Steady state concentrations of extracellular metabolites in yeast Saccharomyces cerevisiae in anaerobic chemostat at D = 0.1 h-1 on minimal medium

Dynamics of extracellular metabolites (glc, pyr, suc, lac, gly, ac, etoh, fum, mal, cit, including loss of akg, g3p, 2pg, 3pg, r5p, f6p, g6p, 6pg) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.

Dynamics of intracellular metabolites (pyr, suc, fum, mal, akg, pep, g3p, 2pg, 3pg, cit, r5p, f6p, g6p, 6pg, ATP, ADP, AMP, UTP, GTP, inosine, NAD+, IMP, UDP, NADP+, CTP, AdenyloSuccinate, NADPH, trehalose) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines,
...

Dynamics of macromolecules (total RNA) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.

This assay describes the determination of concentrations and ratio of metabolites of adenine nucleotides (NAD and NADH).
These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.

Contributor: Stefan Stagge

Assay type: Metabolomics

Technology type: Initial Rate Experiment

Snapshots: No snapshots

This assay describes the determination of concentrations and ratio of metabolites of ubiquinones (oxidised and reduced form).
These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.

We will compare two different procedures to extract ATP from yeast cells: Standard kit procedure (hot Tris/EDTA) and Serrano procedure (cold perchloric acid). In addition we have tested different condition as it turned out that some are important.

No description specified

Contributor: Walter Glaser

Assay type: Metabolomics

Technology type: Imaging

Snapshots: No snapshots

No description specified
No description specified

In this experiment we glucose-pulsed an E. faecalis cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catqabolism of E. faecalis

Contributor: Martijn Bekker

Assay type: Metabolomics

Technology type: Progressive Curve Experiment

Snapshots: No snapshots

In this experiment we glucose-pulsed an L. lactiss cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catabolism of E. L. lactis

Contributor: Martijn Bekker

Assay type: Metabolomics

Technology type: Enzymatic Activity Measurements

Snapshots: No snapshots

No description specified

Measurements of acetone, butanol, acetate, butyrate and ethanol taken during dynamic shift (pH 5.8, 5.5, 5.3, 5.1, 4.9, 4.7, 4.5) and at steady state (pH 5.7, 5.5, 5.3, 5.1, 4.9, 4.7, 4.5).

extracellular metabolite concentrations measured by 1H-NMR

Contributor: Hanna Meyer

Assay type: Metabolomics

Technology type: NMR

Snapshots: No snapshots

intracellular metabolite measured by GC/MS and LC/MS

Contributor: Hanna Meyer

Assay type: Metabolomics

Technology type: NMR

Snapshots: No snapshots

This Excel template is the general (master) template for any type of metabolomics data. It can be used as it is, or extended and modified to create a more specific templates for particular technologies and assay types.

Contributor: Katy Wolstencroft

Assay type: Metabolomics

Technology type: Technology Type

Snapshots: No snapshots

In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose.

Contributor: Araz Zeyniyev

Assay type: Metabolite Profiling

Technology type: Inductively Coupled Plasma Mass Spectrometry

Snapshots: No snapshots

No description specified

Contributor: John Raedts

Assay type: Metabolomics

Technology type: Technology Type

Snapshots: No snapshots

This assay is for method development to quantify intra- and extra-cellular metabolites on T. brucei 427 bloodstream form using isotope ratio based MS technique with 13C-labelled E. coli extract

Intracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.

Metabolite profiling on T. brucei exposed to methylene blue has been carried out using LC-MS to investigate metabolic changes caused by oxidative stress

26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei exposed to methylene blue have been absolutely quantified using isotope ratio based MS technique.

26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei under pH stress (pH8.7) have been absolutely quantified using isotope ratio based MS technique.

Extracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.

Glucose transporter mutants were analyzed under aerobic and aerobic conditions in batch cultures with glucose as substrate. Acetate formation rates and glucose consumption rates were measured, as well as extracellular cAMP concentrations.

Temperature degradation of BPG, GAP and DHAP

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

Experimental data for the conversion of 3PG to F6P and the gluconeogenic pathway intermediates

No description specified

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

BPG degradation at 70C

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

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