Amino acid depletion profile of S. solfataricus during growth on 1 % casaminoacids Version 1
ExploreSulfolobus solfataricus P2 was cultivated on 1 % Caseinhydrolysate. Samples of the culture supernatants were taken at regular intervalls and analyzed by GC-MS. To evaluate the stability of amino acids under cultivation conditions an additional non-inoculated control culture was also analyzed.
SEEK ID: https://fairdomhub.org/data_files/1589?version=1
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Amino acid depletion & secreted compounds.xlsx
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Created: 19th Jan 2017 at 11:41
Last updated: 5th Jun 2017 at 23:13
Last used: 21st Nov 2019 at 17:39
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Related items
Projects: SulfoSys, PSYSMO, SulfoSys - Biotec
Institutions: University of Braunschweig
Projects: SulfoSys - Biotec
Institutions: University of Braunschweig
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012)
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Programme: e:Bio
Public web page: http://www.sulfosys.com/
Integrated systems biology approach including transcriptome, metabolome, proteome analyses and modelling to elucidate amino acid degradation in S. solfataricus P2.
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To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
Person responsible: Jacqueline Wolf
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Intracellular and extracellular metabolome analysis
Contributor: Jacqueline Wolf
Assay type: Metabolite Profiling
Technology type: Gas Chromatography Mass Spectrometry
Snapshots: No snapshots
