Amino acid depletion profile of S. solfataricus during growth on 1 % casaminoacids Version 1
ExploreSulfolobus solfataricus P2 was cultivated on 1 % Caseinhydrolysate. Samples of the culture supernatants were taken at regular intervalls and analyzed by GC-MS. To evaluate the stability of amino acids under cultivation conditions an additional non-inoculated control culture was also analyzed.
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Amino acid depletion & secreted compounds.xlsx
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Meina Neumann-Schaal, Julia Hofmann
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Views: 3101 Downloads: 125
Created: 19th Jan 2017 at 11:41
Last updated: 5th Jun 2017 at 23:13
Last used: 27th Jun 2022 at 07:53
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Version 1 Created 19th Jan 2017 at 11:41 by Jacqueline Wolf
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Institutions: University of Braunschweig
Projects: SulfoSys - Biotec
Institutions: University of Braunschweig
https://orcid.org/0000-0002-9671-5398
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Web page: http://www.fona.de/en/14276
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
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Integrated systems biology approach including transcriptome, metabolome, proteome analyses and modelling to elucidate amino acid degradation in S. solfataricus P2.
Submitter: Jacqueline Wolf
Studies: Comparison of Sulfolobus solfataricus P2 grown on caseinhydrolysate and ...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
Submitter: Jacqueline Wolf
Investigation: Amino acid degradation in Sulfolobus solfataric...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
Intracellular and extracellular metabolome analysis
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Assay type: Metabolite Profiling
Technology type: Gas Chromatography Mass Spectrometry
Investigation: Amino acid degradation in Sulfolobus solfataric...
