Calculation of amino acid uptake rates Version 3
The calculation of amino acid uptake rates for cells of Sulfolobus solfataricus P2 grown on caseinhydrolysate was performed based on the relative consumption of individual amino acids from the growth medium and the previously published absolute concentration of amino acids in the used growth medium.
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Calculation of amino acid uptake rates.ods.ods
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Created: 12th Oct 2016 at 09:09
Last updated: 5th Jun 2017 at 21:13
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Version 3 (latest) Created 26th Oct 2016 at 14:55 by Helge Stark
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Projects: SulfoSys - Biotec
Institutions: University of Braunschweig
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Web page: http://www.fona.de/en/14276
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
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To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
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Intracellular and extracellular metabolome analysis
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