Calculation of amino acid uptake rates Version 3
The calculation of amino acid uptake rates for cells of Sulfolobus solfataricus P2 grown on caseinhydrolysate was performed based on the relative consumption of individual amino acids from the growth medium and the previously published absolute concentration of amino acids in the used growth medium.
SEEK ID: https://fairdomhub.org/data_files/1463?version=3
Filename:
Calculation of amino acid uptake rates.ods.ods
Format: Spreadsheet
Size: 43.1 KB
Export PNG
Creators and SubmitterViews: 3467 Downloads: 138
Created: 12th Oct 2016 at 09:09
Last updated: 5th Jun 2017 at 21:13
TagsThis item has not yet been tagged.
AttributionsNone
Version History
Version 3 (latest) Created 26th Oct 2016 at 14:55 by Helge Stark
Error uploading previous version -_-
Related items
Projects: SulfoSys - Biotec
Institutions: University of Braunschweig
e:Bio - Innovations Competition Systems Biology
Projects: SulfoSys - Biotec, SBEpo - Systems Biology of Erythropoietin
Web page: http://www.fona.de/en/14276
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012) ...
Programme: e:Bio
Public web page: http://www.sulfosys.com/
Integrated systems biology approach including transcriptome, metabolome, proteome analyses and modelling to elucidate amino acid degradation in S. solfataricus P2.
Submitter: Jacqueline Wolf
Studies: Comparison of Sulfolobus solfataricus P2 grown on caseinhydrolysate and ...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
Submitter: Jacqueline Wolf
Investigation: Amino acid degradation in Sulfolobus solfataric...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
Intracellular and extracellular metabolome analysis
Submitter: Jacqueline Wolf
Assay type: Metabolite Profiling
Technology type: Gas Chromatography Mass Spectrometry
Investigation: Amino acid degradation in Sulfolobus solfataric...
Growth on D-glucose served as reference scenario
Submitter: Jacqueline Wolf
Biological problem addressed: Genome Scale
Investigation: Amino acid degradation in Sulfolobus solfataric...
