intracellular metabolites
intracellular metabolite measured by GC/MS and LC/MS
SEEK ID: https://fairdomhub.org/assays/146
Experimental Assay
Projects: BaCell-SysMO
Investigation: Multiomics study of Bacillus subtilis under osmotic stress
Study: B. subtilis_SysMo2_Chemostat_growthrate-salt
Assay type: Metabolomics
Technology type: NMR
Organisms: Bacillus subtilis (chemostat)
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Created: 27th Jan 2012 at 20:15
Last updated: 8th Nov 2017 at 15:21
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Projects: BaCell-SysMO
Institutions: University of Greifswald
BaCell-SysMO 2
Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.
Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying
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Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=53
High salinity chemostat cultivation, multiomics sampling (proteome, transcriptome, metabolome, fluxome) and modelling of carbon core metabolism of Bacillus subtilis 168.
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B. subtilis was grown in minimal media in a chemostat at different growth rates (µ= max, µ=0.1, µ=0.4) and in the presence of 1.2M NaCl (µ=0.1) with or without glycinebetaine. Transcriptome, proteome and metabolome were investigated.
Person responsible: Sandra Maass
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intracellular metabolites measured by LC/MS
intracellular metabolite measured by GC/MS
