Fluxome analysis of Bacillus subtilis 168 under osmotic stress

3 chemostat experiments:

each in 4 biological replicates incl. 1 fed with labelled glucose
T = 37°C
pH = 7.1
V_R = 300 mL (dasgip parallel bioreactor system)
V_G = 9 sL/h (0.5 vvm)
M9 Minimal medium + 3,4-dihydroxybenzoate (chelating agent) + 1g/L Glucose
n = 1000 rpm

3 conditions:

"reference" without additional sodium chloride as control
"stress" supplemented with 1.2M sodium chloride
"osmoprotection" supplemented with 1.2M sodium chloride and 1mM glycine betaine (osmoprotectant)

SEEK ID: https://fairdomhub.org/studies/69

Multiomics study of Bacillus subtilis under osmotic stress

Projects: BaCell-SysMO

Michael Kohlstedt

Experimentalists: Michael Kohlstedt, Claudia Korneli, Judith Becker

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Created: 12th Apr 2011 at 13:19

Last updated: 22nd Nov 2011 at 20:12

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