Follow-up to the validation experiments on FMv2, testing candidate mechanisms for high malate and fumarate accumulation in the Arabidopsis double mutant prr7prr9 and its parent accession Col.
In this study, 14CO2 labelling was used to test the rate of carbon assimilation in the dark at the end of the subjective night (starting about ZT21), which is indicative of PEPC activity in forming malate, and the subsequent partitioning of this labelled C into various cellular fractions. The short-period mutant lhycca1 was also tested, with its parent accession Ws.
SEEK ID: https://fairdomhub.org/assays/1661
Projects: Millar group, TiMet
Investigation: Prediction and analysis of phenotypes in the Arabidopsis clock mutant <i>prr7 prr9</i> using the Framework Model v2 (FMv2)
Study: Test of FMv2, follow-on: mechanisms of malate/fumarate accumulation
Assay type: Metabolite Profiling
Technology type: Technology Type
Organisms: Arabidopsis thaliana : Col-0 wild type (wild-type / wild-type), Arabidopsis thaliana : prr7-3 prr9-1 (T-DNA insertion PRR9;T-DNA insertion PRR7 / 28h circadian rhythm), Arabidopsis thaliana
Created: 8th Apr 2022 at 19:49
Last updated: 15th Apr 2022 at 12:35