Studies
What is a Study?Filters
Here is a collection of examples of possibilities for API communication in R
Literature data used in the Seaton et al. 2017 study; data processing by Daniel Seaton.
Experimental data reported in the Seaton et al. 2017 study; data processing by Alex Graf. Part of the EU FP7 TiMet project.
Submitter: Andrew Millar
Investigation: Photoperiodic control of the Arabidopsis proteo...
Assays: Photoperiod proteomics
Literature data and associated scripts analysed in the Seaton et al. 2017 study; data processing by Daniel Seaton.
Submitter: Andrew Millar
Investigation: Photoperiodic control of the Arabidopsis proteo...
Assays: Aryal et al, 2011, metabolic labelling of Cyanothece protein synthesis, Estimation of rates of translation and turnover from proteomics datasets, Martin et al, 2012, Ostreococcus N15 labelling proteomics data
Data analysis and modelling scripts and results for the Seaton et al. 2017 study, from Daniel Seaton.
Submitter: Andrew Millar
Investigation: Photoperiodic control of the Arabidopsis proteo...
Assays: Translational coincidence model
This stores: Processed data files Links to raw data files Links to repositories containing applied workflows
Submitter: Malte Herold
Investigation: Multi -omics reveal lifestyle of acidophile, mi...
Assays: Experimental methods, Genomics, Proteomics, RNAseq
s-core/ s-core+ network peeling is a methodology to identify cores of weighted complex networks.
Submitter: Eivind Almaas
Investigation: Development of methods for comparing gene co-ex...
Assays: Application of developed network methodology on human and mouse data.
PGK-GAPDH reactions were studied in vitro at 30 and 70 using yeast or Sso enzymes
SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4b/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4c/simulate
Submitter: Theresa Kouril
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK - GAPDH models, PGK-GAPDH 30, PGK-GAPDH 70
PGK reaction at 30 C. Yeast PGK was incubated at 30 C, in the presence or absence of the ATP recycling system, and the conversion of 3 PG to BPG was followed. SED-ML simulations Fig. 1A: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig1a/simulate
Submitter: Jacky Snoep
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 30C data, PGK 30C model
PGK reaction at 70C. Sulfolobus solfataricus PGK was incubated at 70C in presence and absence of an ATP recycling system. Changes in metabolite concentrations was followed via 31P NMR or enzymatic analyses.
SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3b/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3c/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3d/simulate
Submitter: Theresa Kouril
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 70 data, PGK 70C model
BPG produced with yeast PGK was incubated at 70 C,upon which BPG rapidly dephosphorylates to 3PG. SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig2b/simulate
Submitter: Jacky Snoep
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: BPG degradation, BPG stability analysis
After the removal of the extracellular antibiotic, efflux and inhibition dynamics combine to delay the synthesis of ribosomes in a concentration-dependent manner (panel ii). Colors indicate increasing antibiotic concentration, as shown in panel ii.
SED-ML simulation https://jjj.bio.vu.nl/models/experiments/srimani2017_fig2cii/simulate
Submitter: Dawie van Niekerk
Investigation: Srimani et al (2017) Molecular Systems Biology
Assays: Kinetic model of antibiotic-mediated inhibition of ribosomes
Contains copy number per locus tag at different times of Growth between 0.25h and 96 hours. M. pneumoniae was grown in Batch, cells attached to the bottom of the flask (single cell layer), non stirred, non aerated.
Submitter: Niels Zondervan
Investigation: Modelling of M. pneumoniae metabolism
Assays: Transcriptomics assay of M. pneumoniae at diferent times of growth
Personalized liver function tests: A Multiscale Computational Model Predicts Individual Human Liver Function From Single-Cell Metabolism
Understanding how liver function arises from the complex interaction of morphology, perfusion, and metabolism from single cells up to the entire organ requires systems-levels computational approaches. We report a multiscale mathematical model of the Human liver comprising the scales from single hepatocytes, over representation of ultra-structure and micro-circulation ...
Submitter: Matthias König
Investigation: MM-PLF: Multiscale modeling for personalized li...
Assays: Galactose Modelling
Growth-factor deprived mCFU-E cells (5x106 cells per condition) and BaF3-EpoR cells (1x107 cells per condition) were stimulated with different Epo doses and absolute concentrations were determined for pEpoR (B), pAKT (C), ppERK (D). The scale for pS6 (E) was estimated in arbitrary units. GTP-Ras (F) and ppERK were determined upon stimulation with indicated, color-coded Epo doses. pEpoR was analyzed by immunoprecipitation followed by immunoblotting, GTP-Ras was analyzed after pulldown using a ...
Submitter: Jay Moore
Investigation: Metabolism of Streptomyces coelicolor (SysMO ST...
Assays: Metabolic pathway curation
Genotype: Wildtype (M145E) Medium: Phosphate-limited (F134)
Submitter: Jay Moore
Investigation: Metabolism of Streptomyces coelicolor (SysMO ST...
Assays: Online/offline measurements, metabolomics, proteomics, transcriptomics
Submitter: Theresa Kouril
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Assays: Cell free extract activity measurements: L-fuc/d-glc, Metabolic model of Sulfolobus solfataricus, Proteome analysis: d-fuc / l-glu, RNA sequencing:l-fuc/d-glu, intracellular metabolome analysis: l-fucose vs d-glucose
Submitter: Ron Henkel
Investigation: Hands-on: Model Management in SEEK
Submitter: Jan-Willem Veening
Investigation: Chromosome segregation and cell division in Str...
Here you will find guidelines for creating MIAPE compliant proteomics data files as well as examples and links to online tools and resources
Submitter: Katy Wolstencroft
Investigation: Creating data sheet template for 'omics data
Assays: Proteomics Template (gel electrophoresis), Proteomics Templates (Mass spectrometry)
Here you will find guidelines for creating MAGE-TAB compliant transcriptomics data files as well as examples and links to online tools and resources.
Transcriptional response to a sudden increase in extracellular ligand (hormone), for the six network designs of (A). The transcriptional response is taken to equal the ratio ReNrL/Retotal, i.e., the fraction of REs attaching ligand-bound NR. The ligand concentration was increased from 0 to 0.005 nM and maintained constant at the latter level. The observation that design 6 is higher than all other designs at long times is robust for parameter changes up to a factor of 3.
Submitter: Dawie van Niekerk
Investigation: Kolodkin et al (2010) Molecular Systems Biology...
Determination of essential amino acids for Streptococcus pyogenes M49
Lactic acid bacteria generally use homolactic fermentation for generation of ATP. Here we studied the role of Arginine and Glutamine metabolism on the general physiology of the lactic acid bacteria Streptococcus pyogenes. A deletion mutant of glnA (glutamine synthetase) has been constructed in the S. pyogenes M49 591 background. The glnA mutant strain shows decreased growth in low glutamine and excess glutamate conditions and no growth at all in low glutamine and low glutamate conditions. An arcA ...
Submitter: Antje Sieg
Investigation: Amino acid metabolism of four LAB species: Stre...
Assays: Characterization of flux distribution of S. pyogenes M9 wild type and th...
The reconstruction of the metabolic networks is done by sequence comparison with already annotated genomes of L. lactis, L. plantarum, B. subtilis and E. coli
Here you will find all pre-liminary data
Pyruvate kinase (PYK, EC 2.7.1.40) is a key step in glycolysis converting phosphoenolpyruvate into pyruvate. The activity of PYK is activator-dependent, with the allosteric activation mostly being due to fructose-1,6-bisphosphate (FBP).
Submitter: Stefan Henrich
Investigation: The Attic
Assays: literature values for allosteric regulation of pyruvate kinase