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Genome wide marker frequency analysis on new strains to image genetic loci in Streptococcus pneumoniae
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Created: 19th Jul 2016 at 10:32
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Projects: Noisy-Strep
Institutions: University of Groningen
Roles: Project Coordinator
Expertise: Microbiology, Genetics, Molecular Biology, Single Cell analysis, Bacillus subtilis, Bacterial Cell Biology, Molecular microbiology, Medical microbiology, Streptococcus pneumoniae
Tools: Microbiology, Biochemistry, Genetics, Genetic modification, Transcriptomics, Fluorecence based reporter gene analyses/single cell analyses, Molecular biology techniques (RNA/DNA/Protein)
The Veening lab is interested in phenotypic bi-stability in Streptococcus pneumoniae and its importance in virulence of this human pathogen.
Bistable switches are the key elements of the regulatory networks governing cell development, differentiation and life-strategy decisions. Transcriptional noise is a main determinant that causes switching between different states in bistable systems. By using the human pathogen Streptococcus pneumoniae as a model bacterium, we will investigate how transcriptional fidelity and processivity influence (noisy) gene expression and participate in bistability. To study this question, we will use both
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Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=163
Organisms: Streptococcus pneumoniae
Snapshots: No snapshots
Submitter: Jan-Willem Veening
Assay type: Experimental Assay Type
Technology type: Next generation sequencing
Snapshots: No snapshots
Investigation: Chromosome segregation and cell division in Str...
Study: Genome wide marker frequency analysis on new st...
Organisms: No organisms
SOPs: No SOPs
Data files: DCI15, MK453, MK454, Whole genome marker frequency analysis on strai..., Whole genome marker frequency analysis on strai..., Whole genome marker frequency analysis on strai...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Investigations: Chromosome segregation and cell division in Str...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Investigations: Chromosome segregation and cell division in Str...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Investigations: Chromosome segregation and cell division in Str...
Strain MK423 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries
...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Investigations: Chromosome segregation and cell division in Str...
Strain MK422 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries
...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Investigations: Chromosome segregation and cell division in Str...
Strain MK350 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries
...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Investigations: Chromosome segregation and cell division in Str...