Whole genome marker frequency analysis on strain MK423
Version 1

Download View content

Strain MK423 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries were prepared using NEBNext Ultra DNA Library Kit for Illumina prior to sequencing on an Illuminia HiSeq 2000 Platform (all performed at the Genomics Core Facility, EMBL, Heidelberg, Germany).

SEEK ID: https://fairdomhub.org/data_files/1431?version=1

Filename: C74M6ACXX_MK423_15s010991-1-1_Aprianto_lane215s010991_sequence.txt  Download

Format: Plain text document

Size: 1.63 GB

help Creators and Submitter

Views: 2960   Downloads: 134

Created: 19th Jul 2016 at 10:26

Last used: 27th Feb 2021 at 10:09

help Attributions


Related items

Powered by
Copyright © 2008 - 2020 The University of Manchester and HITS gGmbH