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Targeted disruption and over expression of critical enzymes

SilicoTryp

Key enzymes of critical points in the pathways will be targeted for disruption by the generation of RNAi cell lines and lines which drive tetracycline-regulatable ectopic over expression of either wild type enzyme or, if appropriate, dominant-negative or mis-targeted mutants of these. In all cases perturbed lines will be analysed with respect to the mRNA, protein or enzymatic activities of other components of the subsystem, this being directed iteratively by the predictions from systems modelling. ...

Submitter: Federico Rojas

Investigation: Perturbing the biological system: developmental...

Assays: Generation of RNAi cell lines in T.brucei brucei 2T1 cell line, Induction of RNA interference in T.brucei brucei 2T1 cell line

Snapshots: No snapshots

Created: 2nd May 2012 at 15:30

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