Assays
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Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model validation
RobOKoD algorithm was, designed then implemented as part of a study in RobOKoD: microbial strain design for (over)production of target compounds. (http://fairdomhub.org/publications/236). It was used to generate a strain of e.coli for producing butanol, that was then compared to an experimental strain. It was shown to perform better than similar methods (OptKnock, and RobustKnock).
Submitter: Natalie Stanford
Biological problem addressed: Model Analysis Type
Investigation: Designing a new way to predict engineering stra...
OptKnock algorithm was used as part of a study in RobOKoD: microbial strain design for (over)production of target compounds. (http://fairdomhub.org/publications/236). It was used to generate a strain of e.coli for producing butanol, that was then compared to an experimental strain.
Submitter: Natalie Stanford
Biological problem addressed: Model Analysis Type
Investigation: Designing a new way to predict engineering stra...
RobustKnock algorithm was used as part of a study in RobOKoD: microbial strain design for (over)production of target compounds. (http://fairdomhub.org/publications/236). It was used to generate a strain of e.coli for producing butanol, that was then compared to an experimental strain.
Submitter: Natalie Stanford
Biological problem addressed: Model Analysis Type
Investigation: Designing a new way to predict engineering stra...
Submitter: Jay Moore
Biological problem addressed: Metabolic Network
Investigation: Metabolism of Streptomyces coelicolor (SysMO ST...
Time-dependent simulations of the dynamic switch between acidogenesis and solventogenesis based on the metabolic network and pH-dependent regulation of the enzymes.
Submitter: Sara Jabbari
Biological problem addressed: Metabolic Network
Investigation: The effect of pH upon the metabolic shift in Cl...
Steady state study of the effect of altering gene regulation on yields of end-products, focusing on butanol.
Submitter: Sara Jabbari
Biological problem addressed: Gene Expression
Investigation: The effect of pH upon the metabolic shift in Cl...
Theoretical analysis of hypothetical sigma factor competition. Based on the model 'transcription factor competition' possible dynamics of sigma factor competition are simulated and analysed using Lineweaver-Burk representations.
Submitter: Ulf Liebal
Biological problem addressed: Gene Expression
Investigation: The transition from growing to non-growing Baci...
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.
Submitter: Ulf Liebal
Biological problem addressed: Gene Expression
Investigation: The transition from growing to non-growing Baci...
The dynamic model describes response of yeast metabolic network on metabolic perturbation (i.e. glucose-pulse). One compartmental ODE-based model of yeast anaerobic metabolism includes: glycolysis, pentose phosphate reactions, purine de novo synthesis pathway, purine salvage reactions, redox reactions and biomass growth. The model describes metabolic perturbation of steady state growing cells in chemostat.
Submitter: Maksim Zakhartsev
Biological problem addressed: Metabolic Network
Investigation: Kinetic analysis of metabolic system using tran...
Submitter: Sebastian Henkel
Biological problem addressed: Model Analysis Type
Investigation: Steady state studies for different oxygen avail...
Submitter: Sebastian Henkel
Biological problem addressed: Model Analysis Type
Investigation: Steady state studies for different oxygen avail...
- Comparison of metabolic flux distribution in carbon core metabolism (EMP, PPP, TCA) of Bacillus subtilis under 3 different conditions: "salt-free" reference, "stress" chemostat, "osmoprotected" chemostat.
- Model created using OpenFLUX and Microsoft Excel
- Model computed using MatLAB
Submitter: Michael Kohlstedt
Biological problem addressed: Metabolic Network
Investigation: Multiomics study of Bacillus subtilis under osm...
Using PCA, three components, beam size 8. Clustering via MCL from Biolayout Express 3D
Data is taken from "Genome-Wide Gene Expression Analysis of the Switch between Acidogenesis and Solventogenesis in Continuous Cultures of Clostridium acetobutylicum." Grimmler et al. 2011 DOI: 10.1159/000320973
Submitter: Sebastian Curth
Biological problem addressed: Gene Expression
Investigation: The effect of pH upon the metabolic shift in Cl...
Pyruvate formate-lyase (PFL) is an important enzyme in the metabolic pathway of lactic acid bacteria (LAB) and is held responsible for the regulation of the shift between homolactic acid to mixed acid fermentation. PFL catalysis the reversible reaction of acetyl-CoA and formate into pyruvate and CoA. A glycyl radical, who is regenerated within the reaction, is involved; therefore, PFL works only under strictly anaerobic conditions. For its activation, the C-terminal domain has to bind to the ...
Submitter: Stefan Henrich
Biological problem addressed: Model Analysis Type
Investigation: The Attic
Study: Pyruvate formate-lyase (PFL)
Metabolic network of S. pyogenes including primary metabolism, polysaccharide metabolism, purine and pyrimidine biosoynthesis, teichoic acid biosynthesis, fatty acid and phospholipid bioynthesis, amino acid metabolism, vitamins and cofactors
Submitter: Jennifer Levering
Biological problem addressed: Metabolic Network
Investigation: Investigation of glycolysis and pyruvate branch...
Metabolic network of Enterococcus faecalis including primary metabolism, polysaccharide metabolism, purine and pyrimidine biosoynthesis, teichoic acid biosynthesis, fatty acid and phospholipid bioynthesis, amino acid metabolism, vitamins and cofactors
Submitter: Nadine Veith
Biological problem addressed: Metabolic Network
Investigation: Investigation of glycolysis and pyruvate branch...
Submitter: Sebastian Curth
Biological problem addressed: Model Analysis Type
Investigation: General Method Development
Using Taverna for mining and MATLAB for conversion into specific formats (cytoscape, SBTOOLBOX2)
Submitter: Sebastian Curth
Biological problem addressed: Model Analysis Type
Investigation: Modular Model Building
Study: Reactome Analysis
Cytoscape based analysis and yED based representation of clostridial Reactomes
Submitter: Sebastian Curth
Biological problem addressed: Model Analysis Type
Investigation: Modular Model Building
Study: Reactome Analysis
Submitter: Sebastian Curth
Biological problem addressed: Model Analysis Type
Investigation: Modular Model Building
Study: Automated Model Building
Submitter: Sebastian Curth
Biological problem addressed: Model Analysis Type
Investigation: Modular Model Building
Study: Automated Model Building
The model describes the behaviour of E. coli in a stationary chemostat with different oxygen availability.
Submitter: Michael Ederer
Biological problem addressed: Metabolic Network
Investigation: Steady state studies for different oxygen avail...
Using TFinfer2 to analyse data from "Characterization of MG1655 and mutant strains under conditions of glucose excess and limitation"
Submitter: Botond Cseke
Biological problem addressed: Model Analysis Type
Investigation: Analysis of the glucose transport in Escherichi...
Submitter: Katy Wolstencroft
Biological problem addressed: Model Analysis Type
Investigation: Yeast Glycolytic Oscillations
The main input is the ENA review paper (Function and Regulation of the Saccharomyces cerevisiae ENA Sodium ATPase System, Ruiz&Ariño 2007) and the papers referenced. Another source are the papers linked from the ENA page of SGD http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ENA1
Submitter: Falko Krause
Biological problem addressed: Gene Expression
Investigation: ENA1 Transcription in Saccharomyces cerevisiae
A boolean network was created using booleannet (after experimenting with Squad and CellNetAnalyzer). This network can be simulated and visualized using additional software components that will be part of the pyMantis CMS that is developed by the Translucent project.
Submitter: Falko Krause
Biological problem addressed: Gene Regulatory Network
Investigation: ENA1 Transcription in Saccharomyces cerevisiae