Projects: BaCell-SysMO
Institutions: University of Erlangen-Nuernberg
Expertise: Microbiology, Molecular Biology, Bacillus subtilis, regulation of gene expression, carbon metabolism, Clostridium, carbon catabolite regulation in Gram positive bacteria, mRNA, overflow metabolism in Bacillus
Tools: Genetic modification, cultivation, northern blot analysis, Molecular biology techniques (RNA/DNA), Optimal experimental design, absolute quantification, qRT-PCR, reporter gene analyses, Western blot analyses, microbiology techniques
Projects: Noisy-Strep
Institutions: University of Groningen
Projects: Noisy-Strep
Institutions: University of Groningen
Expertise: Microbiology, Genetics, Molecular Biology, Single Cell analysis, Bacillus subtilis, Bacterial Cell Biology, Molecular microbiology, Medical microbiology, Streptococcus pneumoniae
Tools: Microbiology, Biochemistry, Genetics, Genetic modification, Transcriptomics, Fluorecence based reporter gene analyses/single cell analyses, Molecular biology techniques (RNA/DNA/Protein)
The Veening lab is interested in phenotypic bi-stability in Streptococcus pneumoniae and its importance in virulence of this human pathogen.
Projects: BaCell-SysMO
Institutions: University of Erlangen-Nuernberg
Expertise: Molecular Biology, Bacillus subtilis, functional protein expression, protein-protein interactions, microscopy, Bacterial Cell Biology, carbon catabolite regulation in Gram positive bacteria
Tools: Chromatography, Fluorescence and confocal microscopy, Protein chemical methods (protein overproduction, quantitative western blot analysis, Dynamic modelling, 2-D Gel Electrphoresis, Immunofluorescence, Cell culture, Western blot analyses, interaction analysis techniques especially SPR measurements
Projects: BaCell-SysMO
Institutions: University of Erlangen-Nuernberg
Expertise: Bacillus subtilis, functional protein expression, bacterial metabolism, carbon metabolism, Bacterial Cell Biology, Protein-DNA-interaction, Microbiology/ Protein chemistry/ Molecular Biology, Cell physiology, regulation of gene expression, quantative biology
Tools: Microbiology, Biochemistry and protein analysis, Cell biology, Model organisms, Chromatography, Molecular biology techniques (RNA/DNA/Protein), DNA, surface plasmon resonance spectroscopy, reporter gene analysis
Projects: SUMO
Institutions: University of Amsterdam
Expertise: Transcriptomics, Bacillus subtilis, Escherichia coli, stress responses
Tools: Chemostats, HPLC, phage transduction
Projects: BaCell-SysMO
Institutions: University of Greifswald
Expertise: Bioinformatics, Bacillus subtilis
Tools: Microarray analysis
I am pursuing my PhD at Prof. Volker's Lab in the Department of Functional Genomics, EMA Universitat Greifswald, Germany. I am working on the general stress responses mediated by SigB and the prediction of SigB regulon members using the Random forest algorithm.
Projects: BaCell-SysMO
Institutions: University of Greifswald
I am PhD student at Prof.Uwe Voelker lab in Department of Functional Genomics. My area of research is microbial functional genomics in particular analysing the whole transcriptome(by microarray and other molecular biolology methods) of B.subtilis under various stress conditions. I use QconCAT strategy for absolute quantification of carbon metabolic enzymes via MRM(multiple reaction monitoring) by LC-MS/MS. I also perofrm experiments for understanding of dynamics of SigmaB network for modelling.
Projects: BaCell-SysMO
Institutions: University of Stuttgart
Expertise: Microbiology, Biochemistry, Mathematical modelling, Bacillus subtilis, Mathematical modelling of biosystems and bioprocesses, stress responses, Systems Biology, Nonlinear Dynamics, carbon metabolism, Signalling networks, Metabolic Networks
Tools: Computational and theoretical biology, ODE, Matlab, Mathematica, Fermentation, Chromatography, continuous cultivation, Enzyme assay, Computational Systems Biology, Deterministic models, Dynamic modelling, fed-batch cultivation
I am a biologist in the lab of Prof. Reuss at the University of Stuttgart and I am working in the field of biotechnology and mathematical modelling.
Projects: BaCell-SysMO
Institutions: University Medical Centre Groningen
The main area of my expertise concerns protein sorting and secretion in Gram-positive bacteria, such as Bacillus subtilis and Staphylococcus aureus.
The Gram-positive bacterium B. subtilis is well known for its high capacity to secrete proteins into the extracellular milieu, which has led to its exploitation as a "cell factory" for secreted proteins. Nevertheless, the secretion of heterologous proteins of pharmaceutical importance is frequently inefficient. This applied problem has been a major ...
Projects: BaCell-SysMO
Institutions: University of Greifswald
I started to work with B. subtilis during my diploma thesis in Marburg, analyzing the gene expression pattern during sporulation and their control by the four sporulation sigma factors. This work was continued during my PhD thesis in Greifswald. In collaboration with Prof. Bremer and Prof. Marahiel in Marburg we also studied additional adaptation processes of B. subtilis, like the adaptation to low temperatur and high osmolarity. I am now working as a staff scientist in Prof. Völkers lab in ...
Projects: BaCell-SysMO
Institutions: University of Groningen
Expertise: Genetics, Molecular Biology, Microarray analysis, Bacillus subtilis, phenotypic heterogeneity, gene regulation, stress responses, protein secretion, functional protein expression, microscopy, fluorescence protein fusions (transcriptional and translational), localisation studies
Tools: Genetic modification, Transcriptomics, Microarray analysis, Fluorecence based reporter gene analyses/single cell analyses, Site-directed mutagenesis, Fluorescence microscopy, Flow cytometry, Immunofluorescence, transposon mutagenesis, Molecular biology techniques (RNA/DNA/Protein), DNA affinity chromatography, EMSA
PhD student. Analyzing CcpA affinity to cre boxes (catabolite responsive elements) and response of B. subtilis to membrane protein overproduction stress.
Projects: BaCell-SysMO
Institutions: University of Greifswald
Expertise: Microbiology, Molecular Biology, Bacillus subtilis, stress responses, functional protein expression, carbon metabolism
Tools: Molecular Biology, Biochemistry and protein analysis, Proteomics, Proteomics (2D-PAGE), 2-D Gel Electrphoresis, GeLC-MS/MS, metabolic labeling, absolute quantification, AQUA
I'm Post-Doc in the lab of Prof. Becher at the University of Greifswald. I'm working on the relative and absolute protein quantitation using gel-based and mass-spectrometric methods.
Projects: BaCell-SysMO
Institutions: University of Goettingen
Expertise: Microbiology, Genetics, Molecular Biology, Bacillus subtilis, regulation of gene expression, protein-protein interactions, Mycoplasma pneumoniae, Cell physiology, protein-RNA interactions
Tools: Microbiology, Molecular Biology, Biochemistry and protein analysis, Genetic analysis, Genetic modification, Proteomics (2D-PAGE), mutant strain generation, Chemical cross-linking, SubtiWiki, SPINE, bacterial two-hybrid system
Projects: BaCell-SysMO
Institutions: University of Groningen
Expertise: Microbiology, Genetics, Molecular Biology, Bacillus subtilis, translational control of gene expression, sporulation, phenotypic heterogeneity, bistability, gene regulation, stress responses, Signal transduction in Gram-negative bacteria; Synthetic Microbiology; Single cell gene expression; Regulatory networks; biochemistry; histidine ki..., regulation of gene expression, Systems Biology
Tools: Microbiology, Genetics, Molecular Biology, Genetic analysis, Genetic modification, Model organisms, Single Cell analysis, PCR, Fluorecence based reporter gene analyses/single cell analyses, Molecular biology techniques (RNA/DNA), time lapse microscopy, Time-lapse fluorescence microscopy Flow cytometry
Projects: BaCell-SysMO
Institutions: University of Rostock
Expertise: Mathematical modelling, Bacillus subtilis, Deterministic modelling of gene regulation networks, stress responses, Systems Biology, sensitivity analysis, Dynamics and Control of Biological Networks, Parameter estimation
Tools: Biochemistry, Computational and theoretical biology, ODE, Matlab, linux, Stochastic models, Deterministic models, Dynamic modelling
Modelling of the general stress response activation cascade of sigB in B. subtilis in response to starvation.
Creator: Joerg Stuelke
Submitter: Leif Steil
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
List of protein identified proteins in SPINE experiment.
Creators: Joerg Stuelke, Uwe Voelker
Submitter: Leif Steil
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Joerg Stuelke
Submitter: Leif Steil
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Table describing the catabolite repression of β-xylosidase by different carbon sources(glucose, sorbitol, fructose, maltose, glycerol. mannitol) in various mutants of CcpA cofactors (HprK, crh)
Creator: Joerg Stuelke
Submitter: Leif Steil
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Recent studies revealed the unsuspected complexity of the bacterial transcriptome but its systematic analysis across many diverse conditions remains a challenge. Here we report the condition-dependent transcriptome of the prototype strain B. subtilis 168 across 104 conditions reflecting the bacterium's life-styles. This data set composed of 269 tiling array hybridizations allowed to observe ~85% of the annotated CDSs expressed in the higher 30% in at least one hybridization and thus provide an ...
Creators: Leif Steil, Ulrike Mäder, Colin Harwood, Rick Lewis, Ruben Mars, Priyanka Nannapaneni, Praveen kumar Sappa, Joerg Stuelke, Jan Maarten van Dijl, Michael Hecker, Uwe Voelker
Submitter: Leif Steil
Investigations: Redefining the Complete Transcriptome of Bacill...
Studies: Transcriptome analysis of glucose starvation in..., Transcriptome of continuously stressed B. subtilis, Transcriptome of shocked B. subtilis cells
Assays: Tiling Array analysis of glucose strarved B. su..., Tiling Array analysis of shocked B. subtilis cells, Tiling array analysis of continuous growth stre...
Abstract (Expand)
Authors: Frederik M Meyer, Matthieu Jules, Felix M P Mehne, Dominique Le Coq, Jens J Landmann, Boris Görke, Stéphane Aymerich,
Date Published: 14th Oct 2011
Publication Type: Not specified
PubMed ID: 22001508
Citation: