Tools: The Membrane Enzymology group focuses on the molecular mechanisms of transport and signalling across biological membranes. In other words: How do m..., engineering principles and approaches from molecular biology and synthetic chemistry are applied. Confocal microscopy and various spectroscopic met...
Bert Poolman is professor in biochemistry and program director of the Centre for Synthetic Biology. His research focuses on the elucidation of the mechanisms by which signals are transduced and small molecules are translocated across cellular membranes. Cells are reengineered for the production of correctly folded membrane proteins, and methods are developed to reconstitute complex molecular assemblies in synthetic membranes and to analyze their functional and structural properties. The in vitro
I'm Post-Doc in the lab of Prof. Becher at the University of Greifswald. I'm working on the relative and absolute protein quantitation using gel-based and mass-spectrometric methods.
Expertise: quantative biology, Cell physiology, regulation of gene expression, Microbiology/ Protein chemistry/ Molecular Biology, Protein-DNA-interaction, Bacterial Cell Biology, carbon metabolism, bacterial metabolism, functional protein expression, Bacillus subtilis
Tools: surface plasmon resonance spectroscopy, reporter gene analysis, DNA, Molecular biology techniques (RNA/DNA/Protein), Chromatography, Model organisms, Cell biology, Biochemistry and protein analysis, Microbiology
Expertise: carbon catabolite regulation in Gram positive bacteria, Bacterial Cell Biology, microscopy, protein-protein interactions, functional protein expression, Bacillus subtilis, Molecular Biology
Tools: interaction analysis techniques especially SPR measurements, Cell culture, Western blot analyses, Immunofluorescence, 2-D Gel Electrphoresis, Dynamic modelling, quantitative western blot analysis, Fluorescence and confocal microscopy, Protein chemical methods (protein overproduction, Chromatography
Institutions: Helmholtz Centre for Infection Research Braunscheig
Expertise: microscopy, fluorescence protein fusions (transcriptional and translational), localisation studies, protein secretion, functional protein expression, stress responses, gene regulation, phenotypic heterogeneity, Bacillus subtilis, Microarray analysis, Molecular Biology, Genetics
Tools: DNA affinity chromatography, EMSA, Molecular biology techniques (RNA/DNA/Protein), transposon mutagenesis, Flow cytometry, Immunofluorescence, Fluorescence microscopy, Site-directed mutagenesis, Fluorecence based reporter gene analyses/single cell analyses, Microarray analysis, Transcriptomics, Genetic modification
PhD student. Analyzing CcpA affinity to cre boxes (catabolite responsive elements) and response of B. subtilis to membrane protein overproduction stress.