Data files

A dummy file used to test programmatic download from SEEK.

test file

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Main page at NCBI for the salmon genome. Contains download links to annotation GFF, genome sequence FASTA, etc.

RNAseq analysis of S. solfataricus grown on either L-fucose or D-glucose

Activity assays in cell free extracts of S. solfataricus grown on either L-fucose or D-glucose and activity assays with recombinant proteins

Comparative GC-MS based metabolomics of S. solfataricus growing on either L-fucose or D-glucose. CoA derivatives were analysed via HPLC-MS

Comparative proteomics of S. solfatricus grown on either L-fucose or D-glucose.

Publications and patents from the last 6 years

List of publications from 2006 till 2016. Note: I retired from the University of Amsterdam in 2010

3rd ERASysAPP – EXCHANGE Day: Networking and Info Day for research projects of the first and second ERASysAPP calls

just cell counts, the area of the grains is separate.

after shankra, saving the images that could not be analysed earlier. here they are simply treated by imagej change in brightness/contrast. the same can also be done with imagemagic.

Strain MK423 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries ...

Strain MK422 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries ...