Polyglot European Scientist. I thrive working in interdisciplinary environments combining the study of enzyme reactions and mechanisms with bioinformatics, molecular modelling, automated data analysis and data stewardship.
Projects: PoLiMeR - Polymers in the Liver: Metabolism and Regulation
Institutions: University of Groningen
I work as a project manager for the Innovative Training Network PoLiMeR - Polymers in the LIver: Metabolism and Regulation funded by the EU. In addition I am a project manager for the UMCG Research BV where I support scientist in the pre-award phase with writing their proposals and in the post-award phase with managing their awarded projects.
Projects: SysMO-LAB, SysMilk, IMOMESIC
Institutions: Wageningen University & Research, VU University Amsterdam
https://orcid.org/0000-0003-3929-0423
Since August 2008 I am professor in Systems Biology at the VU University Amsterdam. My Systems Bioinformatics group focusses on systems biology with a special focus on integrative bioinformatics. It aims at forming bridges between the classical bottom-up approaches in systems biology and the more data-driven approaches in classical bioinformatics. We combine experimental, modeling and theoretical approaches to study cellular physiology, with an emphasis on metabolic networks.
Institutions: University of Stavanger
Physical chemist with expertise in experimental kinetics, molecular biology, and mathematical modeling.
Projects: SilicoTryp
Institutions: University of Heidelberg
Professor of Biochemistry at the Centre of Biochemistry of Heidelberg University, teaching biochemistry for medical and biology students Research focus is the trypanothione redox metabolism of African trypanosomes (Trypanosoma brucei). The work is funded within the Collaborative Research Centre 544 on "Control of Tropical Infectious Diseases" of the German Research Foundation
Projects: SilicoTryp
Institutions: University of Heidelberg
Activity (initial rates) of Gre2p were measured followng NADPH absorbance at different substrate [hexane-2,5-dione] concentrations. Initial rates are calculated (mM/s) and plotted as a function of [hexane-2,5-dione] to allow fitting with a enzyme kinetic model, e.g. Michaelis Menten. These are the raw data obtained directly from the Synergy UV-Vis Spectrophotometer.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
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Activity (initial rates) of Gre2p were measured followng NADPH absorbance at different substrate [hexane-2,5-dione] concentrations. Initial rates are calculated (mM/s) and plotted as a function of [hexane-2,5-dione] to allow fitting with a enzyme kinetic model, e.g. Michaelis Menten. This is a file that can be opened with the Origin Software for analysis and contains data copied in manually from the xlsx.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Activity (initial rates) of Gre2p were measured followng NADPH absorbance at different substrate [NDK] concentrations. Initial rates are calculated (mM/s) and plotted as a function of [NDK] to allow fitting with a enzyme kinetic model, e.g. Michaelis Menten. This is a file that can be opened with the Origin Software for analysis and contains data copied in manually from the xlsx.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
This is the Jupyter Notebook to allow editing and working with the code.
Design an initial rate experiment in four steps
This script aims to help you design high-quality initial rate experiments. It uses the Michaelis-Menten equation to simulate data for an enzyme with unknown enzyme reaction parameters. Four steps are needed: 1. Estimate the enzyme reaction parameters
Input: estimates of Km and vmax
and indicate the enzyme concentration concentration you are planning to use. If you have no ...
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
SOP describing how to perform activity measurements (inital rates) with Gre2p in multiwell plates (96w) using an UV-Vis Plate reader (Synergy H1).
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
