Comparison of Kcat values model and values from literature. Model values are based on Vmax enzyme parameters (maximum activity per enzyme molecule). Literature values are largely based on whole cell enzyme extract assays and do not take into account allosteric control. In addition activity is measured at varying time points and varying conditions. The error based on differences in enzyme concentrations at different time points and the error in protein copy number measurements is taken into account in the comparison. In addition Sensitivity analysis show the importance of different Vmax values on flux through glycolysis.
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Created: 1st May 2017 at 10:54
Last updated: 14th Jan 2019 at 16:00