Assays

Short Name: SPv10T2Analysis-GCMS Assay Class: WET Assay Type: GCMS Title: Analysis of SPv1.0 T2 plants Description: Analysis of pheromone content in T2 generation of SPv1.0 plants pISA Assay creation date: 2021-11-01 pISA Assay creator: ElenaMG Lab manager: DO Sample collection protocol: Leaf samples were collected at 4 weeks after transplant for young stage and at 7 weeks for adult stage (early flowering). Samples were collected between 4 and 6 pm, frozen in liquid nitrogen immediately after ...

Assay: ASPv10T3Analysis-GCMS Short Name: SPv10T3Analysis-GCMS Assay Class: WET Assay Type: GCMS Title: Analysis of SPv1.0 T3 plants Description: Analysis of pheromone content in T3 generation of SPv1.0 plants pISA Assay creation date: 2021-11-01 pISA Assay creator: ElenaMG Lab manager: DO Sample collection protocol: Leaf samples were collected at 4 weeks after transplant for young stage and at 7 weeks for adult stage (early flowering). Samples were collected between 4 and 6 pm, frozen in liquid ...

Short Name: SPv10EaDActAnalysis-GCMS Assay Class: WET Assay Type: GCMS Title: Transient expression in Nicotiana benthamiana leaves of the moth pheromone pathway with and without EaDAct Description: The effect of EaDAct in pheromone production was studied by agroinfiltrating in Nicotiana benthamiana leaves the first two enzymes (AtrD11 and HarFAR) with or without the last enzyme EaDAct. P19 was also agroinfiltrated as negative control. pISA Assay creation date: 2019-12-08 pISA Assay creator: ElenaMG ...

Short Name: TransientSPv11andSPv12-GCMS Assay Class: WET Assay Type: GCMS Title: Transient expression of constructs for SxPv1.1 and SxPv1.2 Description: The constructs for SxPv1.1 and SxPv1.2 were tested transiently in Nicotiana benthamiana leaves. Pheromone content was analysed via GC-MS pISA Assay creation date: 2020-07-23 pISA Assay creator: ElenaMG Lab manager: DO Sample collection protocol: Leaf samples were collected at 4 weeks after transplant for young stage and at 7 weeks for adult stage ...

Files connected to investigation: _I_T21_SXPsysbio

Files connected to study: _S_P1_SPv10T0andT1

Files connected to study: _S_P1_SPv10T2andT3

Short Name: SP10T0Analysis-GCMS Assay Class: WET Assay Type: GCMS Title: Analysis of SPv1.0 T0 plants Description: Stable transformation of Nicotiana benthamiana plants with the three enzymes for moth pheromone production (AtrD11, HarFAR, EaDAct) expressed constitutively pISA Assay creation date: 2021-11-01 pISA Assay creator: ElenaMG Lab manager: DO Sample collection protocol: Leaf samples were collected at 4 weeks after transplant for young stage and at 7 weeks for adult stage (early flowering). ...

Short Name: SP10T1Analysis-GCMS Assay Class: WET Assay Type: GCMS Title: Analysis of SPv1.0 T1 plants Description: Analysis of pheromone content in T1 generation of SPv1.0 plants pISA Assay creation date: 2021-11-01 pISA Assay creator: ElenaMG Lab manager: DO Sample collection protocol: Leaf samples were collected at 4 weeks after transplant for young stage and at 7 weeks for adult stage (early flowering). Samples were collected between 4 and 6 pm, frozen in liquid nitrogen immediately after ...

Liver samples from Atlantic cod males. Total RNA isolated using TRI Reagent (Fekadu Yadetie). Sequencing performed with Illumina Stranded mRNA (sequencing poly-A mRNA) at Genomic Core Facility at UiB/Haukeland (contact person Rita Holdhus).

The data sets are submitted to the ArrayExpress repository, and will be linked here.

Untargeted lipidomics analysis was performed on plasma and liver samples of four male fish from each group (n = 4) at Per Bruheim’s lab at NTNU, Norway, using Liquid Chromatography Hybrid Quadrupole Mass Spectrometry (UPLC-HDMS).

Dear SEEK users, this Assay is just an example Excel sheet for intracellular metabolites concentration measurements performed using cell culture growing in chemostat

From the "data accessibility" section of Life-stage associated remodeling of lipid metabolism regulation in Atlantic salmon. (Publication):

Supplementary files have been deposited to datadryad.org under the accession: https://doi.org/10.5061/dryad.j4h65. Raw RNA-Seq data have been deposited into European Nucleotide Archive (ENA) under the project Accession no. PRJEB24480.

Dead links 2022-06-29 (the Shiny ...

Short Name: 06_tr_rep-transrate Assay Class: DRY Assay Type: transrate Title: Cultivar representative transcriptome transrate Description: Transrate on cultivar specific representatives, GFF merged as a reference for CRRB pISA Assay creation date: 2020-03-11 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:

Short Name: cdhit_3cvs-GFFmerged Assay Class: DRY Assay Type: GFFmerged Title: cdhit-est and cdhit-2d on 3cv and ITAG/PGSC Description: cdhit-est and cdhit-2d on 3cv and ITAG/PGSC pISA Assay creation date: 2020-03-09 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:

Short Name: 01_GC_content-count Assay Class: DRY Assay Type: count Title: GC content and sequence length Description: A, C, T, G content and sequence length pISA Assay creation date: 2020-03-09 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data: ../input/path_to_files.txt

Leaf number at flowering data from literature for prr7 prr9 and Col wild-type plants under long photoperiods and short photoperiods

Seedling hypocotyl data from literature for prr7 prr9 and Col wild-type plants under various photoperiods

Biomass and metabolites in Col0 (WT) and prr7prr9, with and without exogenous gibberellins (GA)

Luciferase reporter gene assay for circadian period of seedlings in constant light, for Col0 (WT) and prr7prr9, with and without exogenous gibberellins (GA). Supplementary Figure 11f in Chew et al., _in Silico _Plants.

Raw and processed data, together with circadian period analysis and summary statistics, are available from BioDare.ed.ac.uk: choose https://biodare.ed.ac.uk/experiment ("Browse Public Resources" on the Login screen), then you can link to https://biodare.ed.ac.uk/robust/ShowExperiment.action?experimentId=3838, ...