Assays
What is an Assay?Filters
100414H1
| Property | Value |
|---|---|
| BioDare ID | 13982674508951 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
At ZT0 dawn is dim light (6umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.
Purpose
Double dawn experiment to compare to Mark Stitt's results
Comments
Pedro is not able to record changes in ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet double dawns
100414H2
| Property | Value |
|---|---|
| BioDare ID | 13983251032563 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Partner experiment: 100414H1
Purpose
Double dawn experiment to compare to Mark Stitt's results
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet double dawns
130313H1: 14L14D x 4 LL x 3 (T-28)
| Property | Value |
|---|---|
| BioDare ID | 13637871113032 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 14L 14D Experimental conditions: 14L14Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
Wrong imaging script was used (90mins) for T-28. 5mins, 10mins and 10mins of L was cut out ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
200213H1: 15L5D x4 LL x3 (T-20)
| Property | Value |
|---|---|
| BioDare ID | 13618962781900 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle.
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
270213H1: 10L10Dx4 LLx3 (T-20)
| Property | Value |
|---|---|
| BioDare ID | 13624982192162 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
130212H1: 5L15Dx4 LLx3 (T-20)
| Property | Value |
|---|---|
| BioDare ID | 13615471409903 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
1.) 2H of L were cut short at the end of the first day of experimental condition (3L17D). 2.) ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
060313H1: 5L15Dx4 LLx3 (T-20)
| Property | Value |
|---|---|
| BioDare ID | 13631017726171 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
130313H2: 12F-12Dx4 FFx3
| Property | Value |
|---|---|
| BioDare ID | 13639713266200 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Far-Red)
Purpose
Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
060313H2: 12F-12Dx4 FFx3
| Property | Value |
|---|---|
| BioDare ID | 13631095320942 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Far-Red)
Purpose
Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
200213H3: 12B-12Dx4 BB3
| Property | Value |
|---|---|
| BioDare ID | 13619780272531 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)
Purpose
Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
130213H3: 12R-12Dx4 RRx3
| Property | Value |
|---|---|
| BioDare ID | 13618784212904 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)
Purpose
Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
270213H3: 12R-12Dx4 RRx3
| Property | Value |
|---|---|
| BioDare ID | 13630953803730 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)
Purpose
Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
AT0080: 4x18:6-DD
| Property | Value |
|---|---|
| BioDare ID | 12730527312073 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 18L:6D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
Pedro prepared by editing groups from previous template
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
AT0043: 4x 12:12-DD
| Property | Value |
|---|---|
| BioDare ID | 12730739255199 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 12:12 blue+red into constant darkness
Purpose
testing acute light responses at dawn and dusk
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
AT0078: 4x6:18-DD
| Property | Value |
|---|---|
| BioDare ID | 12729899933214 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 6L:18D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
Effects of GA on clock in WT and prr9prr7
| Property | Value |
|---|---|
| BioDare ID | 3838 |
| Author | Daniel Seaton |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Purpose
To test whether GA has any effect on circadian clock period in the WT, and whether this effect is altered in the prr9prr7 mutant.
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Chew Yin Hoon
Study: Chew et al. (2022)
Kim et al. (2005); CO, FT; 16L:8D; WT, elf3
| Property | Value |
|---|---|
| BioDare ID | 13790787578160 |
| Author | Kim, W.Y. |
| Institution | Ohio State University |
| License | CC_BY |
Description
Purpose
The circadian clock regulates many aspects of plant development, including hypocotyl elongation and photoperiodic induction of flowering. ZEITLUPE (ZTL) is a clock-related F-box protein, and altered ZTL expression causes fluence rate-dependent circadian ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Kim, W.Y.
Study: Unpublished
Kim2007 ZTL in blue light
| Property | Value |
|---|---|
| BioDare ID | 13228247820844 |
| Author | Kim WY |
| Institution | Ohio State University |
| License | CC_BY |
Description
Purpose
time courses of TOC1, GI and ZTL proteins in 12:12
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Kim, W.Y.
Study: Unpublished
Ito 2007 wt LD PRR9 protein
| Property | Value |
|---|---|
| BioDare ID | 13227547204752 |
| Author | Ito S |
| Institution | Nagoya University |
| License | CC_BY |
Description
Purpose
PRR9 mRNA and protein were measured under the same conditions. This file has PRR9 protein only.
Comments
This PRR9 protein profile rises more slowly than the FLAG-tagged PRR9 protein in Nakamichi Plant Cell 2010.
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Ito, S.
Study: Unpublished
Sawa et al. (2007); CO; 16L:8D; WT, gi
| Property | Value |
|---|---|
| BioDare ID | 13790763084422 |
| Author | Sawa, M. |
| Institution | Scripps Research Institute |
| License | CC_BY |
Description
Purpose
Precise timing of CONSTANS (CO) gene expression is necessary for day-length discrimination for photoperiodic flowering. The FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1), and GIGANTEA (GI) proteins regulate CO transcription in Arabidopsis. We demonstrate ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Sawa, M.
Study: Unpublished
Ito et al. (2008); GI, FKF1, CDF1, CO, FT; 16L:8D, 10L:14D; WT, toc1, prr5, toc1;prr5
| Property | Value |
|---|---|
| BioDare ID | 13790751359110 |
| Author | Ito, S. |
| Institution | Nagoya University |
| License | CC_BY |
Description
Purpose
In Arabidopsis thaliana, many circadian clock-associated genes have been identified. Among them, the eveningexpressed TOC1 (TIMING OF CAB EXPRESSION 1) gene plays a role by forming a transcriptional feedback core loop ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Ito, S.
Study: Unpublished
Niwa et al. (2007); GI, FKF1, CDF1, CO, FT mRNA; 10L:14D; WT, cca1;lhy, toc1;cca1;lhy
| Property | Value |
|---|---|
| BioDare ID | 13790739219191 |
| Author | Niwa Y. |
| Institution | Nagoya University |
| License | CC_BY |
Description
Purpose
In Arabidopsis thaliana, the flowering time is regulated through the circadian clock that measures day-length and modulates the photoperiodic CO–FT output pathway in accordance with the external coincidence model. ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Niwa Y.
Study: Unpublished
Nakamichi et al. (2007); FKF1, CDF1, CO, FT; 16L:8D, 10L:14D; WT, prr7;5, prr9;7, cca1;lhy, cca1;lhy;prr7;5
| Property | Value |
|---|---|
| BioDare ID | 13790777856363 |
| Author | Nakamichi, N. |
| Institution | Nagoya University |
| License | CC_BY |
Description
Purpose
Photoperiodism allows organisms to measure daylength, or external photoperiod, and to anticipate coming seasons. Daylength measurement requires the integration of light signal and temporal ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Nakamichi Norihito
Study: Unpublished
Matsushika2000_wt_LD_PRR975_mRNA
| Property | Value |
|---|---|
| BioDare ID | 13228202468812 |
| Author | Matsushika A |
| Institution | Nagoya University |
| License | CC_BY |
Description
Purpose
typical time course of PRR9, PRR7, PRR5 in 12:12
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Matsushika A
Study: Unpublished
Nakamichi_2010_draft: PRR9,PRR7,PRR5
| Property | Value |
|---|---|
| BioDare ID | 13401436566946 |
| Author | Norihito Nakamichi |
| Institution | RIKEN Plant Science Center |
| License | CC_BY |
Description
Literature data from: 'PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock.' by: Norihito Nakamichi.
An interlocking transcriptional-translational feedback loop of clock-associated genes is thought ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Nakamichi Norihito
Study: Unpublished
Song et al. (2012); WT, fkf1, COox, COox;fkf1 and COoxCDF1ox in SD and LD at 22oC.
| Property | Value |
|---|---|
| BioDare ID | 13227587445347 |
| Author | Song, Y.H. |
| Institution | University of Washington |
| License | CC_BY |
Description
Literature data from: 'FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering' by: Song, Y.H..
CDF1 and FKF1 have previously been shown to regulate CO mRNA. This data highlights that they ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Song, Y.H.
Study: Unpublished
Song et al. (2012); WT, fkf1, COox, COox;fkf1 and COoxCDF1ox in SD and LD at 22oC.
| Property | Value |
|---|---|
| BioDare ID | 13228237712731 |
| Author | Song, Y.H. |
| Institution | University of Washington |
| License | CC_BY |
Description
Literature data from: 'FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering' by: Song, Y.H..
CDF1 and FKF1 have previously been shown to regulate CO mRNA. This data highlights that they ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Song, Y.H.
Study: Unpublished
The TIME FOR COFFEE Gene Maintains the Amplitude and Timing of Arabidopsis Circadian Clocks
| Property | Value |
|---|---|
| BioDare ID | 13227577822113 |
| Author | Hall A |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'The TIME FOR COFFEE gene maintains the amplitude and timing of Arabidopsis circadian clocks' by: Hall A.
Plants synchronize developmental and metabolic processes with the earth’s 24-h rotation ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Hall A
Study: Unpublished
Mizoguchi2002_wt_gi_LL_LHY_CCA1
| Property | Value |
|---|---|
| BioDare ID | 13228428214032 |
| Author | Mizoguchi Tsuyoshi |
| Institution | John Innes Centre |
| License | CC_BY |
Description
Literature data from: 'LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis' by: Mizoguchi Tsuyoshi.
comparison of wt and gi-3 (Ler) in LL
Purpose
LL time courses for LHY, CCA1 mRNA for wt, gi; soil
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Tsuyoshi Mizoguchi
Study: Unpublished
FLOWERING LOCUS C Controls Temperature Response of the Arabidopsis Clock
| Property | Value |
|---|---|
| BioDare ID | 13227500410222 |
| Author | Edwards KD |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'FLOWERING LOCUS C mediates natural variation in the high-temperature response of the Arabidopsis circadian clock' by: Edwards KD.
Authors have analyzed FLC alleles in near-isogenic lines and induced mutants ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Kieron D Edwards
Study: Unpublished