Assays

What is an Assay?
349 Assays visible to you, out of a total of 372

100414H1

Property Value
BioDare ID 13982674508951
Author Yvette Wilson
Institution
License

Description

At ZT0 dawn is dim light (6umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in ...

100414H2

Property Value
BioDare ID 13983251032563
Author Yvette Wilson
Institution
License

Description

12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 100414H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

130313H1: 14L14D x 4 LL x 3 (T-28)

Property Value
BioDare ID 13637871113032
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 14L 14D Experimental conditions: 14L14Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

Wrong imaging script was used (90mins) for T-28. 5mins, 10mins and 10mins of L was cut out ...

200213H1: 15L5D x4 LL x3 (T-20)

Property Value
BioDare ID 13618962781900
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle.

Comments

270213H1: 10L10Dx4 LLx3 (T-20)

Property Value
BioDare ID 13624982192162
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

130212H1: 5L15Dx4 LLx3 (T-20)

Property Value
BioDare ID 13615471409903
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

1.) 2H of L were cut short at the end of the first day of experimental condition (3L17D). 2.) ...

060313H1: 5L15Dx4 LLx3 (T-20)

Property Value
BioDare ID 13631017726171
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

130313H2: 12F-12Dx4 FFx3

Property Value
BioDare ID 13639713266200
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Far-Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

060313H2: 12F-12Dx4 FFx3

Property Value
BioDare ID 13631095320942
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Far-Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

200213H3: 12B-12Dx4 BB3

Property Value
BioDare ID 13619780272531
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

130213H3: 12R-12Dx4 RRx3

Property Value
BioDare ID 13618784212904
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

270213H3: 12R-12Dx4 RRx3

Property Value
BioDare ID 13630953803730
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

AT0080: 4x18:6-DD

Property Value
BioDare ID 12730527312073
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 18L:6D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

Pedro prepared by editing groups from previous template

AT0043: 4x 12:12-DD

Property Value
BioDare ID 12730739255199
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 12:12 blue+red into constant darkness

Purpose

testing acute light responses at dawn and dusk

Comments

AT0078: 4x6:18-DD

Property Value
BioDare ID 12729899933214
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 6L:18D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

Effects of GA on clock in WT and prr9prr7

Property Value
BioDare ID 3838
Author Daniel Seaton
Institution University of Edinburgh
License CC_BY

Description

Purpose

To test whether GA has any effect on circadian clock period in the WT, and whether this effect is altered in the prr9prr7 mutant.

Comments

Kim et al. (2005); CO, FT; 16L:8D; WT, elf3

Property Value
BioDare ID 13790787578160
Author Kim, W.Y.
Institution Ohio State University
License CC_BY

Description

Purpose

The circadian clock regulates many aspects of plant development, including hypocotyl elongation and photoperiodic induction of flowering. ZEITLUPE (ZTL) is a clock-related F-box protein, and altered ZTL expression causes fluence rate-dependent circadian ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Kim, W.Y.

Study: Unpublished

Kim2007 ZTL in blue light

Property Value
BioDare ID 13228247820844
Author Kim WY
Institution Ohio State University
License CC_BY

Description

Purpose

time courses of TOC1, GI and ZTL proteins in 12:12

Comments

Ito 2007 wt LD PRR9 protein

Property Value
BioDare ID 13227547204752
Author Ito S
Institution Nagoya University
License CC_BY

Description

Purpose

PRR9 mRNA and protein were measured under the same conditions. This file has PRR9 protein only.

Comments

This PRR9 protein profile rises more slowly than the FLAG-tagged PRR9 protein in Nakamichi Plant Cell 2010.

Sawa et al. (2007); CO; 16L:8D; WT, gi

Property Value
BioDare ID 13790763084422
Author Sawa, M.
Institution Scripps Research Institute
License CC_BY

Description

Purpose

Precise timing of CONSTANS (CO) gene expression is necessary for day-length discrimination for photoperiodic flowering. The FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1), and GIGANTEA (GI) proteins regulate CO transcription in Arabidopsis. We demonstrate ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Sawa, M.

Study: Unpublished

Ito et al. (2008); GI, FKF1, CDF1, CO, FT; 16L:8D, 10L:14D; WT, toc1, prr5, toc1;prr5

Property Value
BioDare ID 13790751359110
Author Ito, S.
Institution Nagoya University
License CC_BY

Description

Purpose

In Arabidopsis thaliana, many circadian clock-associated genes have been identified. Among them, the eveningexpressed TOC1 (TIMING OF CAB EXPRESSION 1) gene plays a role by forming a transcriptional feedback core loop ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Ito, S.

Study: Unpublished

Niwa et al. (2007); GI, FKF1, CDF1, CO, FT mRNA; 10L:14D; WT, cca1;lhy, toc1;cca1;lhy

Property Value
BioDare ID 13790739219191
Author Niwa Y.
Institution Nagoya University
License CC_BY

Description

Purpose

In Arabidopsis thaliana, the flowering time is regulated through the circadian clock that measures day-length and modulates the photoperiodic CO–FT output pathway in accordance with the external coincidence model. ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Niwa Y.

Study: Unpublished

Nakamichi et al. (2007); FKF1, CDF1, CO, FT; 16L:8D, 10L:14D; WT, prr7;5, prr9;7, cca1;lhy, cca1;lhy;prr7;5

Property Value
BioDare ID 13790777856363
Author Nakamichi, N.
Institution Nagoya University
License CC_BY

Description

Purpose

Photoperiodism allows organisms to measure daylength, or external photoperiod, and to anticipate coming seasons. Daylength measurement requires the integration of light signal and temporal ...

Matsushika2000_wt_LD_PRR975_mRNA

Property Value
BioDare ID 13228202468812
Author Matsushika A
Institution Nagoya University
License CC_BY

Description

Purpose

typical time course of PRR9, PRR7, PRR5 in 12:12

Comments

Nakamichi_2010_draft: PRR9,PRR7,PRR5

Property Value
BioDare ID 13401436566946
Author Norihito Nakamichi
Institution RIKEN Plant Science Center
License CC_BY

Description

Literature data from: 'PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock.' by: Norihito Nakamichi.

An interlocking transcriptional-translational feedback loop of clock-associated genes is thought ...

Song et al. (2012); WT, fkf1, COox, COox;fkf1 and COoxCDF1ox in SD and LD at 22oC.

Property Value
BioDare ID 13227587445347
Author Song, Y.H.
Institution University of Washington
License CC_BY

Description

Literature data from: 'FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering' by: Song, Y.H..

CDF1 and FKF1 have previously been shown to regulate CO mRNA. This data highlights that they ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Song, Y.H.

Study: Unpublished

Song et al. (2012); WT, fkf1, COox, COox;fkf1 and COoxCDF1ox in SD and LD at 22oC.

Property Value
BioDare ID 13228237712731
Author Song, Y.H.
Institution University of Washington
License CC_BY

Description

Literature data from: 'FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering' by: Song, Y.H..

CDF1 and FKF1 have previously been shown to regulate CO mRNA. This data highlights that they ...

The TIME FOR COFFEE Gene Maintains the Amplitude and Timing of Arabidopsis Circadian Clocks

Property Value
BioDare ID 13227577822113
Author Hall A
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'The TIME FOR COFFEE gene maintains the amplitude and timing of Arabidopsis circadian clocks' by: Hall A.

Plants synchronize developmental and metabolic processes with the earth’s 24-h rotation ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Hall A

Study: Unpublished

Mizoguchi2002_wt_gi_LL_LHY_CCA1

Property Value
BioDare ID 13228428214032
Author Mizoguchi Tsuyoshi
Institution John Innes Centre
License CC_BY

Description

Literature data from: 'LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis' by: Mizoguchi Tsuyoshi.

comparison of wt and gi-3 (Ler) in LL

Purpose

LL time courses for LHY, CCA1 mRNA for wt, gi; soil

Comments

FLOWERING LOCUS C Controls Temperature Response of the Arabidopsis Clock

Property Value
BioDare ID 13227500410222
Author Edwards KD
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'FLOWERING LOCUS C mediates natural variation in the high-temperature response of the Arabidopsis circadian clock' by: Edwards KD.

Authors have analyzed FLC alleles in near-isogenic lines and induced mutants ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Kieron D Edwards

Study: Unpublished

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