Fermentative Indole Production via Bacterial Tryptophan Synthase Alpha Subunit and Plant Indole-3-Glycerol Phosphate Lyase Enzymes

Abstract:

Indole is produced in nature by diverse organisms and exhibits a characteristic odor described as animal, fecal, and floral. In addition, it contributes to the flavor in foods, and it is applied in the fragrance and flavor industry. In nature, indole is synthesized either from tryptophan by bacterial tryptophanases (TNAs) or from indole-3-glycerol phosphate (IGP) by plant indole-3-glycerol phosphate lyases (IGLs). While it is widely accepted that the tryptophan synthase α-subunit (TSA) has intrinsically low IGL activity in the absence of the tryptophan synthase β-subunit, in this study, we show that Corynebacterium glutamicum TSA functions as a bona fide IGL and can support fermentative indole production in strains providing IGP. By bioprospecting additional bacterial TSAs and plant IGLs that function as bona fide IGLs were identified. Capturing indole in an overlay enabled indole production to titers of about 0.7 g L-1 in fermentations using C. glutamicum strains expressing either the endogenous TSA gene or the IGL gene from wheat.

SEEK ID: https://fairdomhub.org/publications/652

DOI: 10.1021/acs.jafc.2c01042

Projects: INDIE - Biotechnological production of sustainable indole

Publication type: Journal

Journal: Journal of Agricultural and Food Chemistry

Citation: J. Agric. Food Chem. 70(18):5634-5645

Date Published: 11th May 2022

Registered Mode: by DOI

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Citation
Ferrer, L., Mindt, M., Suarez-Diez, M., Jilg, T., Zagorščak, M., Lee, J.-H., Gruden, K., Wendisch, V. F., & Cankar, K. (2022). Fermentative Indole Production via Bacterial Tryptophan Synthase Alpha Subunit and Plant Indole-3-Glycerol Phosphate Lyase Enzymes. In Journal of Agricultural and Food Chemistry (Vol. 70, Issue 18, pp. 5634–5645). American Chemical Society (ACS). https://doi.org/10.1021/acs.jafc.2c01042
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Created: 14th Sep 2022 at 13:01

Last updated: 14th Sep 2022 at 13:06

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