Studies
What is a Study?Filters
Key enzymes of critical points in the pathways will be targeted for disruption by the generation of RNAi cell lines and lines which drive tetracycline-regulatable ectopic over expression of either wild type enzyme or, if appropriate, dominant-negative or mis-targeted mutants of these. In all cases perturbed lines will be analysed with respect to the mRNA, protein or enzymatic activities of other components of the subsystem, this being directed iteratively by the predictions from systems modelling. ...
We have already demonstrated that the key metabolites of polyamine biosynthesis (arginine, ornithine, putrescine and spermidine) can be identified using HILIC chromatography coupled to the Orbitrap mass spectrometer, as can glycine, glutamate and cysteine used in glutathione biosynthesis, glutathionyl spermidine and trypanothione itself. Furthermore the key metabolites of the methionine cycle (methionine, S-adenosyl methionine, decarboxylated S-adenosyl methionine, methylthioadenosine) can all ...
Submitter: Dong-Hyun Kim
Investigation: Metabolite profiling, quantification and flux q...
Assays: Intracellular metabolite concentrations in T. brucei under pH stress
This study aims to establish the optimum conditions and assay methods for measuring ATP levels
Submitter: Martin Valachovic
Investigation: Effect of Benzoic Acid on ATP Levels
Assays: Compare ATP extraction methods
Submitter: Matthew Rolfe
Investigation: Dynamical studies for different oxygen availabi...
Assays: Transcriptional profiling of E. coli during anaerobic to aerobic and aer...
Submitter: Michael Ederer
Investigation: Steady state studies for different oxygen avail...
Assays: ArcA phosphorylation at different aerobiosis levels (steady states), Determination of intracellular redox state by means of NAD/NADH ratio, Determination of intracellular redox state by means of ubiquinones (oxd/..., FNR activity at different aerobiosis levels (steady state), Literature Data from Alexeeva et al., J. Bacteriol., 2000, 2002, 2003, Measurement of cytochrome numbers, Physiological measurements from Sheffield chemostat, Steady State Oxygen Response of E. coli WT and two Electron Transport Ch..., Transcriptional profiling of steady states at different aerobiosis levels
For cells to accurately read out the genomic content, high fidelity during transcription is required. This is mainly established by the accuracy of the active centre of RNA polymerase (RNAP). Based on in vitro experiments with Escherichia coli RNAP it was also suggested that proofreading of transcription via RNA hydrolysis by RNAP may contribute to overall fidelity and processivity. RNAP’s intrinsic cleavage activity is stimulated by the highly conserved Gre factors suggesting that Gre factors ...
Submitter: Jan-Willem Veening
Investigation: Wetlab approach to transcription fidelity
Assays: RNA-Seq
Genotype: Wildtype (M145E) Medium: Phosphate-limited (F134)
Submitter: Jay Moore
Investigation: Metabolism of Streptomyces coelicolor (SysMO ST...
Assays: Online/offline measurements, metabolomics, proteomics, transcriptomics
Multi experimental approach to define the gene expression remodelling under potassium starvation conditions.
Submitter: Falko Krause
Investigation: K+ Starvation in Saccharomyces cerevisiae
Assays: LacZ Reporters, RT-PCR, Time Course Micro Array Experiment
Submitter: Falko Krause
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Assays: No Assays
Annotation retrieval
Submitter: Praveen kumar Sappa
Investigation: Redefining the Complete Transcriptome of Bacill...
Assays: Tiling Array analysis of glucose strarved B. subtilis cells
Growth of B.subtilis in shakeflask at 57°C; 16°C, 37°C(Control) and 1,2M NaCl in SMM.
Submitter: Praveen kumar Sappa
Investigation: Redefining the Complete Transcriptome of Bacill...
Assays: Tiling array analysis of continuous growth stress conditions in SMM
BMM EtOH, 16, 57 SMM NaCl
Submitter: Praveen kumar Sappa
Investigation: Redefining the Complete Transcriptome of Bacill...
Studies on the effect of deletion of genes encoding 14-3-3 proteins on the transcriptional response of yeast cells to potassium starvation.
Submitter: Falko Krause
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Assays: No Assays
Experiments using shake flask cultures to measure dynamics associated with sigB response.
Submitter: Ulf Liebal
Investigation: The transition from growing to non-growing Baci...
Assays: No Assays