Studies
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PGK reaction at 30 C. Yeast PGK was incubated at 30 C, in the presence or absence of the ATP recycling system, and the conversion of 3 PG to BPG was followed. SED-ML simulations Fig. 1A: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig1a/simulate
Submitter: Jacky Snoep
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 30C data, PGK 30C model
PGK reaction at 70C. Sulfolobus solfataricus PGK was incubated at 70C in presence and absence of an ATP recycling system. Changes in metabolite concentrations was followed via 31P NMR or enzymatic analyses.
SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3b/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3c/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3d/simulate
Submitter: Theresa Kouril
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 70 data, PGK 70C model
PGK-GAPDH reactions were studied in vitro at 30 and 70 using yeast or Sso enzymes
SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4b/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4c/simulate
Submitter: Theresa Kouril
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK - GAPDH models, PGK-GAPDH 30, PGK-GAPDH 70
The pilot experiment has been set up in order to develop uniform SOPs for the Sulfosys consortium. It comprises proteomics, transcriptomics, metabolomics as well as enzymatic essays. Cells for all the members of the consortium have been obtained from the same batch fermentations according to fermentation SOP of Sulfosys. The pilot resulted in creating a procedures for all the techniques used in consortium.
Submitter: Pawel Sierocinski
Investigation: Analysis of Central Carbon Metabolism of Sulfol...
Assays: Comparison of proteome of S. solfataricus at 70 and 80C, Comparison of transcriptome of S. solfataricus at 70 and 80C, Enzyme activity tests for S. solfataricus, Fermentation of S. solfataricus at 70 and 80C in a batch fermenter, Intracellular metabolomics of S. solfataricus at 70 and 80C
Here you will find all pre-liminary data
Submitter: Falko Krause
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Assays: Insilico Promotor Anaysis
Does the general proteome of yeast cells change in a strain lacking Trk1,2 transporter? Under which conditions?
Submitter: Falko Krause
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Assays: 2D Gel Analysis
What is the proteome of starved cells. Main characteristics?
Submitter: Falko Krause
Investigation: K+ Starvation in Saccharomyces cerevisiae
Pyruvate formate-lyase (PFL) is an important enzyme in the metabolic pathway of lactic acid bacteria (LAB) and is held responsible for the regulation of the shift between homolactic acid to mixed acid fermentation. PFL catalysis the reversible reaction of acetyl-CoA and formate into pyruvate and CoA. A glycyl radical, who is regenerated within the reaction, is involved; therefore, PFL works only under strictly anaerobic conditions. For its activation, the C-terminal domain has to bind to the ...
Submitter: Stefan Henrich
Investigation: The Attic
Assays: Pyruvate formate-lyase (PFL): literature review, structure analysis and ...