Experiments using shake flask cultures to measure dynamics associated with sigB response.
SEEK ID: https://fairdomhub.org/studies/5
Experimentalists: Praveen Kumar Sappa
Created: 24th Aug 2009 at 18:44
Last updated: 22nd Nov 2011 at 20:12
Expertise: Systems Biology, sensitivity analysis, stress responses, Deterministic modelling of gene regulation networks, dynamics and control of biological networks, parameter estimation, Bacillus subtilis, Mathematical modelling
Modelling of the general stress response activation cascade of sigB in B. subtilis in response to starvation.
Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.
Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying
The objective of this project is an integrated understanding the metabolic, proteomic and genetic network that controls the transition from growth to glucose starvation. This transition is a fundamental ecophysiological response that serves as a scientific model for environmental signal integration and is pivotal for industrial fermentations of Bacillus that occur predominantly under nutrient starvation.
Glucose starvation, Transcriptomics, Proteomics, Metabolomics,Bacillus subtilis,
Snapshots: No snapshots
Studies: B. subtilis Transcription Factor Competition, Batchfermentation exp-starv01_090204, Biphase Batch Fermentation(2009/02/04), Controlled sigmaB induction in shake flask, Transition to starvation in shake flask
Assays: 2D-gelbased analysis of intracellular proteins, Absolute quantification of proteins by the AQUA-technology, B. subtilis Transcription Factor Competition - theoretical interpretation, B. subtilis Transcription Factor Competition - theoretical interpretation, Fermentation-BM5_SysMo, Gene expression(Transcriptome), IPTG induction of sigmaB in BSA115, IPTG induction of sigmaB in BSA115, Relative quantification of proteins by metabolic labeling, Stressosome activation dynamics, metabolome-LCMS
Data sheet generated in Greifswald to measure the response of sigB activity in response to different media composition.
Investigations: The transition from growing to non-growing Baci... and 1 hidden item
Studies: Transition to starvation in shake flask and 1 hidden item
Assays: 2 hidden items