IPTG induction of sigmaB in BSA115

We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.

There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.

SEEK ID: https://fairdomhub.org/assays/129

Modelling Analysis

Ulf Liebal

Projects: BaCell-SysMO

Investigation: The transition from growing to non-growing Bacillus subtilis cells

Study: Controlled sigmaB induction in shake flask

Assay position:

Biological problem addressed: Gene Expression

Organisms: Bacillus subtilis : 168 (wild-type / wild-type) (batch)

help Creators and Submitter
Creators
Not specified
Submitter
Activity

Views: 3342

Created: 2nd Feb 2011 at 15:07

Last updated: 8th Nov 2017 at 15:21

help Tags

This item has not yet been tagged.

Powered by
(v.1.12.1)
Copyright © 2008 - 2022 The University of Manchester and HITS gGmbH

By continuing to use this site you agree to the use of cookies