IPTG induction of sigmaB in BSA115

We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.

There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.

SEEK ID: https://fairdomhub.org/assays/129

Modelling Analysis

Ulf Liebal

Projects: BaCell-SysMO

Investigation: The transition from growing to non-growing Bacillus subtilis cells

Study: Controlled sigmaB induction in shake flask

Assay position:

Biological problem addressed: Gene Expression

Organisms: Bacillus subtilis : 168 (wild-type / wild-type) (batch)

help Creators and Submitter
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Views: 3342

Created: 2nd Feb 2011 at 15:07

Last updated: 8th Nov 2017 at 15:21

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