B. subtilis Transcription Factor Competition - theoretical interpretation
Theoretical analysis of hypothetical sigma factor competition. Based on the model 'transcription factor competition' possible dynamics of sigma factor competition are simulated and analysed using Lineweaver-Burk representations.
SEEK ID: https://fairdomhub.org/assays/34
Experimental assay
Projects: BaCell-SysMO
Investigation: The transition from growing to non-growing Bacillus subtilis cells
Study: B. subtilis Transcription Factor Competition
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: Bacillus subtilis
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Created: 15th Apr 2010 at 12:31
Last updated: 8th Nov 2017 at 14:21
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Institutions: University of Rostock
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Submitter: Praveen kumar Sappa
Studies: B. subtilis Transcription Factor Competition, Batchfermentation exp-starv01_090204, Biphase Batch Fermentation(2009/02/04), Controlled sigmaB induction in shake flask, Transition to starvation in shake flask
Assays: 2D-gelbased analysis of intracellular proteins, Absolute quantification of proteins by the AQUA-technology, B. subtilis Transcription Factor Competition - theoretical interpretation, B. subtilis Transcription Factor Competition - theoretical interpretation, Fermentation-BM5_SysMo, Gene expression(Transcriptome), IPTG induction of sigmaB in BSA115, IPTG induction of sigmaB in BSA115, Relative quantification of proteins by metabolic labeling, Stressosome activation dynamics, metabolome-LCMS
The aim of the study is to test the interplay of the effects of different transcriptional regulators on gene expression during different environmental responses in B. subtilis. For a selected set of transcriptional regulators native proteins are fusion-labelled. The intensity of expression of each reporter protein is measured in the absence of several transcriptional regulators that compete for the RNA polymerase.
