BSA115-transcription inhibition model Version 3
Simulate Model on JWSThe model represents a hypothetical situation in which an anti-sigmafactor reduces sigB efficacy.
SEEK ID: https://fairdomhub.org/models/49?version=3
1 item is associated with this Model:-
bsa_trscrinhib.xml (SBML and XML document - 13.8 KB)
Organism: Bacillus subtilis
Model type: Ordinary differential equations (ODE)
Model format: SBML
Execution or visualisation environment: JWS Online
Model image: No image specified
Contributor and Creators
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Created: 30th Mar 2011 at 14:31
Last updated: 17th Jan 2013 at 18:31
Last used: 16th Aug 2018 at 09:22
AttributionsNone
Related items
Projects: BaCell-SysMO
Institutions: University of Rostock
Modelling of the general stress response activation cascade of sigB in B. subtilis in response to starvation.
BaCell-SysMO 2
Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.
Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying
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Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=53
The objective of this project is an integrated understanding the metabolic, proteomic and genetic network that controls the transition from growth to glucose starvation. This transition is a fundamental ecophysiological response that serves as a scientific model for environmental signal integration and is pivotal for industrial fermentations of Bacillus that occur predominantly under nutrient starvation.
Keywords:
Glucose starvation, Transcriptomics, Proteomics, Metabolomics,Bacillus subtilis,
Snapshots: No snapshots
In this kind of studies sigmaB stress response is induced by the addition of artificial inducers of sigmaB. For example simgaB is downstream of a Pspac promoter and induced by the addition of IPTG.
A ctc::lacZ reporter gene is used to monitor sigmaB activity.
Person responsible: Ulf Liebal
Snapshots: No snapshots
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.
Abstract (Expand)
Authors: Ulf Liebal, Praveen Kumar Sappa, Thomas Millat, Leif Steil, Uwe Voelker, Olaf Wolkenhauer, Homuth Georg
Date Published: 2012
Journal: Mol. BioSyst.
