0804_shake-flask-sigB-starvation
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Data sheet generated in Greifswald to measure the response of sigB activity in response to different media composition.

SEEK ID: https://fairdomhub.org/data_files/6?version=1

Filename: 0804_starvation-sigb-wt.xls

Format: Spreadsheet

Size: 72.5 KB

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Creators and Submitter

Creator

Ulf Liebal

Submitter

Ulf Liebal

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No license - no permission to use unless the owner grants a licence

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Views: 3974 Downloads: 114

Created: 26th Oct 2009 at 14:48

Last updated: 20th May 2014 at 14:19

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Version 1 (earliest) Created 26th Oct 2009 at 14:48 by Ulf Liebal

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People (1)
Programmes (1)
Projects (1)
Investigations (2)
Studies (2)
Assays (1+1)

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Ulf Liebal

Projects: BaCell-SysMO

Institutions: University of Rostock

Expertise: Mathematical modelling, Bacillus subtilis, Deterministic modelling of gene regulation networks, stress responses, Systems Biology, sensitivity analysis, Dynamics and Control of Biological Networks, Parameter estimation

Tools: Biochemistry, Computational and theoretical biology, ODE, Matlab, linux, Stochastic models, Deterministic models, Dynamic modelling

Modelling of the general stress response activation cascade of sigB in B. subtilis in response to starvation.

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SysMO

SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".

The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.

Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...

Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep

Web page: http://sysmo.net/

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BaCell-SysMO

BaCell-SysMO 2 Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.

Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying ...

Programme: SysMO

Public web page: http://www.sysmo.net/index.php?index=53

Organisms: Bacillus subtilis

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Using SEEK and I-S-A structure to integrate experimentation and mathematical modelling

Kinetics on the move - Workshop 2016

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Olga Krebs

Research in Systems Biology involves integrating data and knowledge about the dynamic processes in biological systems in order to understand and model them. By connecting fields such as genomics, proteomics, bioinformatics, mathematics, cell biology, genetics, mathematics, engineering and computer sciences, Systems Biology enables discovery of yet unknown principles underlying the functioning of living cells. At the same time, testable and predictive models of complex cellular pathways and ...

Submitter: Olga Krebs

Studies: Experimental study for hands on session

Assays: How to use templates for FAIR experimental data representation, How to use templates to structure experimental data 222

Snapshots: Snapshot 1, Snapshot 2

Created: 27th May 2016 at 09:35, Last updated: 26th May 2021 at 05:23

The transition from growing to non-growing Bacillus subtilis cells

BaCell-SysMO

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Praveen kumar Sappa

The objective of this project is an integrated understanding the metabolic, proteomic and genetic network that controls the transition from growth to glucose starvation. This transition is a fundamental ecophysiological response that serves as a scientific model for environmental signal integration and is pivotal for industrial fermentations of Bacillus that occur predominantly under nutrient starvation.

Keywords: Glucose starvation, Transcriptomics, Proteomics, Metabolomics,Bacillus subtilis,

Submitter: Praveen kumar Sappa

Studies: B. subtilis Transcription Factor Competition, Batchfermentation exp-starv01_090204, Biphase Batch Fermentation(2009/02/04), Controlled sigmaB induction in shake flask, Transition to starvation in shake flask

Assays: 2D-gelbased analysis of intracellular proteins, Absolute quantification of proteins by the AQUA-technology, B. subtilis Transcription Factor Competition - theoretical interpretation, B. subtilis Transcription Factor Competition - theoretical interpretation, Fermentation-BM5_SysMo, Gene expression(Transcriptome), IPTG induction of sigmaB in BSA115, IPTG induction of sigmaB in BSA115, Relative quantification of proteins by metabolic labeling, Stressosome activation dynamics, metabolome-LCMS